大黄对脓毒症大鼠小肠微循环的影响和机制研究

发布时间：2018-06-16 09:12

本文选题：脓毒症 + 肠粘膜　；参考：《第二军医大学》2013年硕士论文

【摘要】：目的：通过观察脓毒症大鼠小肠粘膜、肠壁血流量和肠组织氧合的改变，探讨大黄保护肠粘膜微循环的可能性及作用机制。方法：选用健康雄性SD大鼠56只，体重220-250g，随机分为对照组、假手术组、脓毒症组和大黄治疗组，采用盲肠结扎穿孔（CLP）脓毒症模型，大黄治疗组在CLP造模前给予大黄（50mg/kg）灌胃，2次/日×2日，其他组给予灌胃等量生理盐水。模型制备后24小时用moorFLPI散斑血流实时成像系统检测小肠粘膜、肠壁的血流量；用ELISA方法测定肠组织匀浆上清液乳酸和丙酮酸浓度并计算乳酸/丙酮酸的比值；用明胶墨汁灌注+HE染色显示肠粘膜毛细血管，并计算微血管面积（MVA）；免疫组化检测肠粘膜毛细血管CD31的表达并计算微血管密度（MVD）。结果：（1）与脓毒症组（2020.3±353.4）比较，正常组（2799.4±370.6，p0.01）、假手术组（2514.2±484.9，p0.01）、大黄治疗组（2653.0±284.0，p0.01）大鼠肠壁血流量较高（血流单位pu）；（2）同样，与脓毒症组肠粘膜血流量（1662.6±462.0）比较，正常组（2548.2±336.5，p0.05）、假手术组（2514.0±565.1，p0.05）、大黄治疗组（2383.2±834.9，，p0.05）血流量较高（血流单位pu）。（3）与正常组（0.52±0.05，p0.01）、假手术组（0.43±0.04，p0.01）和大黄治疗组（0.50±0.02，p0.01）相比，CLP脓毒症模型组（0.64±0.03）小肠组织乳酸/丙酮酸比值增高。（4）与脓毒症组大鼠（3.68±1.13）比较，正常组（6.26±0.69，p0.01）、假手术组（6.04±0.37，p0.01）、脓毒症大黄治疗组（6.73±1.52，p0.01）大鼠的单位面积内肠粘膜血管面积明显较高。（5）与脓毒症组大鼠（41.08±5.90）比较，正常组（59.42±5.43，p0.01）、假手术组（55.08±5.80，p0.01）、脓毒症大黄治疗组（56.87±6.36，p0.01）大鼠肠粘膜单位面积内毛细血管计数明显较高。结论：大黄可以扩张肠粘膜毛细血管、减少血栓形成、保护肠粘膜毛细血管内皮细胞，增加肠粘膜功能毛细血管数量，从而改善脓毒症状态肠粘膜的血供和氧供。
[Abstract]:Aim: to investigate the possibility and mechanism of rhubarb in protecting intestinal mucosal microcirculation by observing the changes of intestinal mucosal and intestinal wall blood flow and intestinal tissue oxygenation in septic rats. Methods: Fifty-six male Sprague-Dawley rats, weighing 220 to 250 g, were randomly divided into three groups: control group, sham operation group, sepsis group and rhubarb treatment group. The model of sepsis was induced by cecal ligation and perforation (CLP). The rhubarb treatment group was given 50 mg / kg rhubarb twice a day 脳 2 days before CLP. The other groups were given the same amount of normal saline. 24 hours after the model was made, the blood flow of intestinal mucosa and intestinal wall was measured by moorFLPI speckle flow real time imaging system, the concentration of lactate and pyruvate in supernatant of intestinal homogenate was measured by Elisa and the ratio of lactate to pyruvate was calculated. Gelatin ink perfusion HE staining was used to show the intestinal mucosal capillaries, and the microvessel area was calculated. The expression of CD31 in intestinal mucosal capillaries was detected by immunohistochemistry and the microvessel density was calculated. Results compared with sepsis group (2020.3 卤353.4), the intestinal wall blood flow in normal group (2799.4 卤370.6), sham operation group (2514.2 卤484.9), rhubarb treatment group (2653.0 卤284.0 p0.01) was higher than that in septic group (blood flow unit putaman2), which was also higher than that in septic group (1662.6 卤462.0). The blood flow was higher in normal group (2548.2 卤336.5), sham operation group (2514.0 卤565.1), rhubarb treatment group (2383.2 卤834.9p0.05) (blood flow unit pu.f.3) and normal group (0.52 卤0.05p 0.01, sham operation group 0.43 卤0.04p0.01) and rhubarb treatment group (0.50 卤0.02p0.01) compared with CLP sepsis model group (0.64 卤0.03) and the ratio of small intestinal tissue to pyruvate increased by 0.64 卤0.03). The comparison between the rats of toxic group (3.68 卤1.13) and the control group (3. 68 卤1. 13), The intestinal mucosal vascular area in the normal group (6.26 卤0.69p 0.01g), the sham-operated group (6.04 卤0.37p0.01g) and the septic rhubarb treatment group (6.73 卤1.52p0.01) was significantly higher than that in the septic group (41.08 卤5.90), and that in the control group was significantly higher than that in the sepsis group (41.08 卤5.90). In the normal group (59.42 卤5.43), the sham operation group (55.08 卤5.80) and the septic rhubarb treatment group (56.87 卤6.36), the capillary count in the intestinal mucosal unit area was significantly higher than that in the sham operation group (55.08 卤5.80) and the septic rhubarb treatment group (56.87 卤6.36). Conclusion: rhubarb can dilate intestinal mucosal capillaries, reduce thrombosis, protect intestinal mucosal capillary endothelial cells, increase the number of intestinal mucosal functional capillaries, and improve blood supply and oxygen supply of intestinal mucosa in septic state.
【学位授予单位】：第二军医大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R459.7

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