网膜素-1与急性脑梗死发病相关性研究
发布时间：2018-07-25 08:13
【摘要】：目的 通过检测急性脑梗死患者与健康对照组的血清网膜素-1表达水平,以及急性脑梗死不同分组及其不同亚型中血清网膜素-1的表达水平,探讨网膜素-1在急性脑梗死发病中所起的作用,并进一步探讨网膜素-1参与脑梗死发病的可能机制。 方法 根据纳入标准和排除标准,连续性收集180例门诊及住院的急性脑梗死患者,另外取健康体检者180例作为对照。根据颈动脉彩超检查将脑梗死患者分为无动脉粥样硬化组、稳定型斑块组及不稳定型斑块组,同时采用牛津郡社区卒中项目(Oxfordshire Community Stroke Project,OCSP)将脑梗死患者分为四个不同的临床类型：1、全前循环脑梗死；2、部分前循环脑梗死3、后循环脑梗死4、腔隙性脑梗死。采用双抗体夹心酶联免疫吸附法(ELISA)测定标本中血清网膜素-1水平.检测急性脑梗死患者及健康体检者空腹血清网膜素-1水平。统计学分析采用SPSS19.0软件,所有实验数据以x±s表示,应用双侧t检验比较人网膜素-1在急性脑梗死患者以及正常对照人群中的差异性表达。应用单因素方差分析来比较人网膜素-1在无动脉粥样硬化组、稳定型斑块组和不稳定型斑块组中的差异性表达；应用单因素方差分析,比较人网膜素-1在不同亚型急性脑梗死患者中的差异性表达,p0.05为差异具有统计学意义。 结果 血清网膜素-1水平在急性脑梗死患者中显著低于健康对照人群(p0.05)；在稳定型斑块组及不稳定型斑块组患者中显著低于无动脉粥样硬化组(p0.05,p0.05),而在稳定型斑块组及不稳定型斑块组之间无显著性差异(p0.05)；在全前循环梗死型、部分前循环梗死型以及后循环梗死型中显著低于腔隙性梗死型(p0.05,p0.05,p0.05),而在全前循环梗死型、部分前循环梗死型以及后循环梗死型之间无显著性差异(p0.05)。 结论 血清网膜素-1水平在急性脑梗死患者中显著低于健康对照组人群；在稳定型斑块组及不稳定型斑块组患者中显著低于无动脉粥样硬化组,而在稳定型板块组及不稳定型斑块组之间无显著性差异；在全前循环梗死型、部分前循环梗死型以及后循环梗死型中显著低于腔隙性梗死型,而在全前循环梗死型、部分前循环梗死型以及后循环梗死型之间无显著性差异。由于血清网膜素-1在急性脑梗死患者及其不同亚型中存在的差异性表达,以及与健康对照人群的显著性差异,可以推测血清网膜素-1将来可能成为一种新的急性脑梗死诊断、严重程度及预后判断的指标。
[Abstract]:Objective to detect the level of serum omentin-1 in patients with acute cerebral infarction and healthy controls, and to detect the expression of omentin-1 in different subgroups and subtypes of acute cerebral infarction. To explore the role of omentin--1 in the pathogenesis of acute cerebral infarction and to further explore the possible mechanism of retinin--1 involved in the pathogenesis of cerebral infarction. Methods 180 consecutive patients with acute cerebral infarction were collected according to the inclusion criteria and exclusion criteria, and 180 healthy persons were taken as control. Patients with cerebral infarction were divided into three groups according to carotid color Doppler ultrasonography: no atherosclerosis group, stable plaque group and unstable plaque group. At the same time, the patients with cerebral infarction were divided into four different clinical types: 1: 1, 2 cases of total anterior circulation infarction, 3 cases of partial anterior circulation cerebral infarction, 4 cases of posterior circulation cerebral infarction and 4 lacunar cerebral infarction using Oxfordshire Community Stroke Project (Oxfordshire Community Stroke Project OCSP). The level of omentin-1 in serum was determined by double antibody sandwich enzyme linked immunosorbent assay (ELISA). Fasting serum omentin-1 levels were measured in patients with acute cerebral infarction and healthy controls. Statistical analysis was performed with SPSS19.0 software, and all experimental data were expressed as x 卤s. The expression of human omentin-1 in patients with acute cerebral infarction and normal controls was compared by using bilateral t test. Univariate analysis of variance was used to compare the differential expression of human omentin-1 in atherosclerotic, stable and unstable plaque groups. The difference of expression of human omentin-1 in different subtypes of acute cerebral infarction was statistically significant. Results the serum level of omentin-1 in patients with acute cerebral infarction was significantly lower than that in healthy controls (p0.05). In stable plaque group and unstable plaque group, it was significantly lower than that in non-atherosclerosis group (p0.05), but there was no significant difference between stable plaque group and unstable plaque group (p0.05), and in total anterior circulation infarction type, there was no significant difference between stable plaque group and unstable plaque group (p0.05). The incidence of partial anterior circulation infarction and posterior circulation infarction was significantly lower than that of lacunar infarction (p0.05), but there was no significant difference among total anterior circulation infarction, partial anterior circulation infarction and posterior circulation infarction (p0.05). Conclusion the serum level of omentin-1 in patients with acute cerebral infarction is significantly lower than that in healthy controls, and that in stable plaque and unstable plaque is significantly lower than that in non-atherosclerosis. However, there was no significant difference between the stable plate group and unstable plaque group, and in the total anterior circulation infarction type, partial anterior circulation infarction type and posterior circulation infarction type were significantly lower than that in the lacunar infarction type, but in the total anterior circulation infarction type. There was no significant difference between partial anterior circulation infarction type and posterior circulation infarction type. Because of the differential expression of serum omentin-1 in patients with acute cerebral infarction and its subtypes, and the significant difference between serum omentin-1 and healthy controls, it can be speculated that serum omentin--1 may become a new diagnosis of acute cerebral infarction in the future. Severity and prognostic criteria.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R743.33
本文编号：2143189
[Abstract]:Objective to detect the level of serum omentin-1 in patients with acute cerebral infarction and healthy controls, and to detect the expression of omentin-1 in different subgroups and subtypes of acute cerebral infarction. To explore the role of omentin--1 in the pathogenesis of acute cerebral infarction and to further explore the possible mechanism of retinin--1 involved in the pathogenesis of cerebral infarction. Methods 180 consecutive patients with acute cerebral infarction were collected according to the inclusion criteria and exclusion criteria, and 180 healthy persons were taken as control. Patients with cerebral infarction were divided into three groups according to carotid color Doppler ultrasonography: no atherosclerosis group, stable plaque group and unstable plaque group. At the same time, the patients with cerebral infarction were divided into four different clinical types: 1: 1, 2 cases of total anterior circulation infarction, 3 cases of partial anterior circulation cerebral infarction, 4 cases of posterior circulation cerebral infarction and 4 lacunar cerebral infarction using Oxfordshire Community Stroke Project (Oxfordshire Community Stroke Project OCSP). The level of omentin-1 in serum was determined by double antibody sandwich enzyme linked immunosorbent assay (ELISA). Fasting serum omentin-1 levels were measured in patients with acute cerebral infarction and healthy controls. Statistical analysis was performed with SPSS19.0 software, and all experimental data were expressed as x 卤s. The expression of human omentin-1 in patients with acute cerebral infarction and normal controls was compared by using bilateral t test. Univariate analysis of variance was used to compare the differential expression of human omentin-1 in atherosclerotic, stable and unstable plaque groups. The difference of expression of human omentin-1 in different subtypes of acute cerebral infarction was statistically significant. Results the serum level of omentin-1 in patients with acute cerebral infarction was significantly lower than that in healthy controls (p0.05). In stable plaque group and unstable plaque group, it was significantly lower than that in non-atherosclerosis group (p0.05), but there was no significant difference between stable plaque group and unstable plaque group (p0.05), and in total anterior circulation infarction type, there was no significant difference between stable plaque group and unstable plaque group (p0.05). The incidence of partial anterior circulation infarction and posterior circulation infarction was significantly lower than that of lacunar infarction (p0.05), but there was no significant difference among total anterior circulation infarction, partial anterior circulation infarction and posterior circulation infarction (p0.05). Conclusion the serum level of omentin-1 in patients with acute cerebral infarction is significantly lower than that in healthy controls, and that in stable plaque and unstable plaque is significantly lower than that in non-atherosclerosis. However, there was no significant difference between the stable plate group and unstable plaque group, and in the total anterior circulation infarction type, partial anterior circulation infarction type and posterior circulation infarction type were significantly lower than that in the lacunar infarction type, but in the total anterior circulation infarction type. There was no significant difference between partial anterior circulation infarction type and posterior circulation infarction type. Because of the differential expression of serum omentin-1 in patients with acute cerebral infarction and its subtypes, and the significant difference between serum omentin-1 and healthy controls, it can be speculated that serum omentin--1 may become a new diagnosis of acute cerebral infarction in the future. Severity and prognostic criteria.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R743.33
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