骨髓间充质干细胞移植修复皮肤创伤

发布时间：2018-09-18 09:50

【摘要】：背景:目前大部分有关间充质干细胞治疗皮肤创伤的实验均使用大鼠、小鼠和兔进行,而应用犬骨髓间充质干细胞治疗皮肤创伤的研究较少。目的:观察犬骨髓间充质干细胞治疗皮肤创面的效果。方法:在4只犬肩胛部、臀部两侧分别做3 cm×3 cm的正方形皮肤创伤,右侧作为实验组,在创面制作后的第1,3天,创口皮下注射异体犬骨髓间充质干细胞悬液;左侧作为对照组,在创面制作后的第1,3天,创口皮下注射间充质干细胞培养基,动态观察创面愈合情况。结果与结论:在第1周时间内,两组创口处都有淡黄色炎性物质存在,炎症反应明显,且对照组炎性物质较实验组多,肉芽组织生长速度慢于实验组;第2周至伤口愈合时间段,创口在形成肉芽组织的基础上开始明显上皮化,主要表现从伤口四周向中央形成新鲜上皮,实验组开始上皮化时间早于对照组,创口面积小于对照组;第3周时,实验组有的创口完全愈合,且愈合后的创面较对照组平整、光滑。实验组细胞移植后8,12 d的创口面积略小于对照组,创口愈合速度稍快于对照组,但差异均无统计学意义。结果表明,犬骨髓间充质干细胞移植具有促进皮肤创面愈合的可能或趋势。
[Abstract]:BACKGROUND: Rats, mice and rabbits have been used in most of the experiments on the treatment of skin wounds, but few studies have been done on the treatment of skin wounds with canine bone marrow mesenchymal stem cells. The wound healing was observed dynamically on the first and third days after the wound was made by injecting allogeneic canine bone marrow mesenchymal stem cell suspension subcutaneously on the right side as the experimental group and on the left side as the control group on the first and third days after the wound was made. During the period of wound healing from the second week to the wound healing period, the wound began to epithelialize markedly on the basis of granulation tissue formation, mainly in the form of fresh around the wound to the center. Epithelium, the experimental group began to epithelialize earlier than the control group, the wound area was smaller than the control group; at the third week, some wounds in the experimental group healed completely, and the wound healed smoothly and smoothly than the control group. The wound area of the experimental group was slightly smaller than the control group at 8 and 12 days after cell transplantation, and the wound healing speed was slightly faster than the control group, but the difference was not statistically significant. The results showed that the transplantation of bone marrow mesenchymal stem cells could promote the healing of skin wounds.
【作者单位】：佛山科学技术学院生命科学与工程学院;
【基金】：国家自然科学基金(31072101) 佛山市科技创新专项资金项目(佛财工[2013]137号) 2016“攀登计划”广东大学生科技创新培育专项资金项目~~
【分类号】：R641

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