间充质干细胞条件培养液对正常成纤维细胞及瘢痕成纤维细胞转化生长因子β产生和信号通路的影响

发布时间：2019-04-10 20:47

【摘要】：背景:已有文献报道,间充质干细胞可通过分泌大量生物活性因子有效减轻损伤的心脏、肺脏、肾脏等器官纤维化的发生。目的:观察骨髓间充质干细胞条件培养液对正常成纤维细胞和瘢痕成纤维细胞的影响及作用机制。方法:制备骨髓间充质干细胞条件培养液,将其作用于正常成纤维细胞和增生性瘢痕成纤维细胞,采用ELISA检测转化生长因子β的分泌水平,RT-PCR检测TGF-β/Smad信号通路相关基因Smad7的表达,比色法检测各组细胞上清液中羟脯氨酸的含量。结果与结论:(1)骨髓间充质干细胞条件培养液对增生性瘢痕成纤维细胞转化生长因子β的分泌、胶原的合成有显著抑制作用(P0.01),并且明显增强Smad7基因的表达(P0.01);(2)骨髓间充质干细胞条件培养液对正常成纤维细胞的影响较小;(3)结果表明,骨髓间充质干细胞条件培养液对增生性瘢痕成纤维细胞具有显著抑制作用,为干细胞防治皮肤瘢痕提供基础理论支持。
[Abstract]:Background: it has been reported that mesenchymal stem cells can effectively reduce the occurrence of fibrosis in injured heart, lung, kidney and other organs by secreting a large number of bioactive factors. Aim: to observe the effect and mechanism of conditioned medium of bone marrow mesenchymal stem cells on normal fibroblasts and scar fibroblasts. Methods: the conditioned medium of bone marrow mesenchymal stem cells was prepared and applied to normal fibroblasts and hypertrophic scar fibroblasts. The secretion of transforming growth factor 尾 (TGF 尾) was detected by ELISA. The expression of Smad7 related to TGF- 尾 / Smad signaling pathway was detected by RT-PCR, and the content of hydroxyproline in the supernatant of each group was detected by colorimetric assay. Results and conclusion: (1) conditioned medium of bone marrow mesenchymal stem cells significantly inhibited the secretion of TGF-尾 and collagen synthesis in hypertrophic scar fibroblasts (P0.01). And significantly increased the expression of Smad7 gene (P0.01); (2) the conditioned medium of bone marrow mesenchymal stem cells had little effect on normal fibroblasts; (3) the results showed that the conditioned medium of bone marrow mesenchymal stem cells had significant inhibitory effect on hypertrophic scar fibroblasts, which provided the basic theory support for the prevention and treatment of skin scar by stem cells.
【作者单位】： 牡丹江医学院医药研究中心;牡丹江医学院红旗医院眼科;牡丹江医学院病理学教研室;牡丹江医学院解剖学教研室;牡丹江医学院红旗医院检验科;
【基金】：黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2015109)~~
【分类号】：R641
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