不同程度弥漫性轴索损伤动物模型建立研究

发布时间：2019-04-13 16:47

【摘要】：目的自制一种弥漫性轴索损伤(DAI)装置,建立不同程度的DAI动物模型。方法选取成年雄性清洁级SD大鼠70只,分为正常对照组(10只)与致伤组(60只),进一步将致伤组分为A、B、C 3组,各20只。运用自制的实验装置致伤,使大鼠的头颅同时产生瞬间超大角加速度和线加速度,在恒定气压下通过不同的旋转往返加速运动次数,造成轻型(A组)、中型(B组)、重型(C组)3种不同程度的大鼠DAI损伤模型,进行行为及病理效应评价。结果随着损伤程度的加重,损伤急性期致伤组大鼠的神经生理反射恢复的时间间隔与苏醒时间均增加(P0.05),致伤7d后致伤组大鼠的神经功能评分降低(P0.05);致伤后14d内A、B、C 3组大鼠死亡率分别为5.0%、25.0%、50.0%。随着损伤程度的加重,DAI病理特征改变更为明显。结论该装置能有效建立不同损伤程度的DAI动物模型。
[Abstract]:Objective to establish the animal model of diffuse axonal injury (DAI) by self-made (DAI) device. Methods 70 adult male SD rats were divided into normal control group (n = 10) and injury group (n = 60). The injured rats were further divided into A, B and C groups with 20 rats in each group. The injury was caused by a self-made experimental device, which made the rat's head produce instantaneous super-angular acceleration and linear acceleration at the same time, and accelerated the movement through different rotations under constant air pressure, resulting in light weight (group A) and medium-sized (group B). Severe (group C) three different degrees of DAI damage models were evaluated for their behavior and pathological effects. Results with the severity of injury, the interval of recovery of neurophysiological reflex and recovery time of rats in acute stage of injury increased (P0.05), and the neurological function score of injured group decreased 7 days after injury (P0.05). Within 14 days after injury, the mortality rates of A, B and C groups were 5.0%, 25.0% and 50.0%, respectively. With the severity of injury, the pathological changes of DAI were more obvious. Conclusion this device can effectively establish the animal model of DAI with different degree of injury.
【作者单位】：重庆医科大学附属儿童医院神经外科/国家重点临床专科;
【基金】：国家临床重点专科资助项目(X3257) 重庆市科委科技攻关应用技术研发类项目(cstc2012gg-yyjs10013)
【分类号】：R651.15;R-332

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