阿霉素磁性介孔氧化硅纳米颗粒对口腔鳞状细胞癌细胞的体外实验研究

发布时间：2018-03-22 06:17

本文选题：阿霉素　切入点：介孔氧化硅纳米颗粒　出处：《口腔医学研究》2017年08期 　论文类型：期刊论文

【摘要】：目的:探讨阿霉素磁性介孔氧化硅纳米颗粒(Doxorubicin-loading Mesoporous Magnetic Silia Nanoparticles,DOX@M-MSNs)对口腔鳞状细胞癌细胞的体外靶向杀伤作用。方法:采用甲基噻唑基四唑(MTT)法,流式细胞仪及激光共聚焦显微镜检测DOX@M-MSNs对CAL-27的体外靶向抗肿瘤效果。结果:单纯磁性介孔氧化硅纳米颗粒表现出非常好的生物稳定性以及生物相容性,尽管浓度为100 mg/L时,细胞存活率仍超过80%。当浓度值有所提升及作用时间延长后,相较于单纯阿霉素原料组,DOX@M-MSNs对于肿瘤细胞的增殖抑制作用明显增强,差异有统计学意义(P0.05)。流式细胞仪检测结果显示DOX@M-MSNs促进肿瘤细胞的凋亡率高于单纯阿霉素,随着药物作用时间延长,其对肿瘤细胞的抑制效果明显增强,凋亡率增高。激光共聚焦显微镜检测结果显示通过外加磁场,磁性区域的DOX@M-MSN释放的DOX于肿瘤细胞的聚集优于非磁性区域。结论:DOX@M-MSNs纳米颗粒能够选择性进入肿瘤细胞内,从而使得阿霉素对肿瘤细胞的毒性有所增强,进而使得其杀伤肿瘤细胞的效果显著提高,外加磁场可以显著地提升输送DOX@M-MSNs进到肿瘤细胞内的效率,进而提高肿瘤抑制效果。
[Abstract]:Objective: to investigate the killing effect of doxorubicin-loading Mesoporous Magnetic Silia DOXR M-MSNs on oral squamous cell carcinoma cells in vitro. Methods: methylthiazolyl tetrazolium tetrazolium tetrazolium (MTT) method was used to investigate the killing effect of doxorubicin-loading (Doxorubicin-loading) on oral squamous cell carcinoma (OSCC) in vitro. Flow cytometry and laser confocal microscopy were used to detect the anti-tumor effect of DOX@M-MSNs on CAL-27 in vitro. Results: the pure magnetic mesoporous silica nanoparticles showed excellent biostability and biocompatibility, even when the concentration was 100 mg/L. The cell survival rate was still more than 80%. When the concentration was increased and the action time was prolonged, the inhibitory effect of DOXR M-MSNs on the proliferation of tumor cells was significantly increased compared with that of DOXR M-MSNs. The results of flow cytometry showed that the apoptosis rate of tumor cells promoted by DOX@M-MSNs was higher than that of adriamycin alone. The results of laser confocal microscopy showed that the DOX released by magnetic region DOX@M-MSN was superior to that of non-magnetic region in the aggregation of tumor cells. Conclusion: the DOX@M-MSN nanoparticles can selectively enter the tumor cells. Thus, the toxicity of adriamycin to tumor cells was enhanced, and the killing effect of adriamycin on tumor cells was significantly improved. The efficiency of DOX@M-MSNs transport into tumor cells could be significantly enhanced by external magnetic field, and then the tumor inhibition effect could be improved.
【作者单位】：佳木斯大学附属第二医院口腔医院颌面外科二科佳木斯大学;嘉兴市第二医院口腔科;佳木斯大学附属第二医院口腔医院牙体牙髓科一科;
【基金】：佳木斯大学研究生科技创新项目(编号:LZZ2015-021) 黑龙江省教育厅科学技术研究项目(编号:12521553)
【分类号】：R739.8

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