舌鳞状细胞癌淋巴结转移与长链非编码RNA HOTAIR的关系探讨

发布时间：2018-03-22 07:32

本文选题：舌鳞状细胞癌　切入点：淋巴结转移　出处：《口腔医学研究》2017年02期 　论文类型：期刊论文

【摘要】：目的:研究HOTAIR在舌鳞状细胞癌中的表达,并探究HOTAIR与舌鳞状细胞癌淋巴结转移的关系。方法:对19例舌鳞状细胞癌患者的癌组织以及其对应的19例癌旁组织进行实时荧光定量PCR检测,检测HOTAIR在癌组织、癌旁组织的相对表达量,把结果与患者的TNM分期进行关联比较,并进一步通过构建沉默HOTAIR表达的CAL27、SCC9细胞系,检测肿瘤细胞侵袭、迁移能力的改变。结果:19对舌鳞状细胞癌组织中,26.3%显示出HOTAI的高表达(P=0.016 90.05),且高表达者均为具有淋巴结转移的患者,而在无淋巴结转移的癌组织中HOTAIR呈现低表达(P0.001),通过RNA干扰技术降低HOTAIR在CAL27及SCC9细胞系中的表达水平,结果发现细胞侵袭(P0.001)和迁移能力(P0.001)受到了抑制。结论:长链非编码RNA HOTAIR在舌鳞状细胞癌淋巴结转移过程中可能发挥促进因子的作用。
[Abstract]:Objective: to study the expression of HOTAIR in squamous cell carcinoma of tongue. Methods: to investigate the relationship between HOTAIR and lymph node metastasis in tongue squamous cell carcinoma. Methods: 19 patients with tongue squamous cell carcinoma and 19 adjacent tissues were detected by real-time fluorescence quantitative PCR, and HOTAIR was detected in cancer tissue. The relative expression level of paracancerous tissues was correlated with the TNM stage of the patients. Furthermore, the invasion of tumor cells was detected by constructing a cell line CAL27, SCC9, which silenced the expression of HOTAIR. Results 26.3% of patients with squamous cell carcinoma of tongue showed high expression of HOTAI (0.016 90.05%), and the patients with high expression were all patients with lymph node metastasis. However, low expression of HOTAIR was found in cancer tissues without lymph node metastasis, and the expression of HOTAIR in CAL27 and SCC9 cell lines was decreased by RNA interference technique. The results showed that cell invasion and migration were inhibited. Conclusion: long chain noncoding RNA HOTAIR may play a role as a promoter in lymph node metastasis of tongue squamous cell carcinoma.
【作者单位】：广州医科大学附属口腔医院·广州口腔病研究所·口腔医学重点实验室;
【基金】：广东省科技计划项目(编号:2013B021800196;20120318025;2012B0320001200)
【分类号】：R739.86

【参考文献】