趋化因子CXCL16及其受体CXCR6在中重度牙周炎患者牙龈组织中的表达

发布时间：2018-03-26 13:49

本文选题：牙周炎　切入点：炎症　出处：《大连医科大学》2014年硕士论文

【摘要】：目的：牙周病是一种慢性感染性疾病，一系列的免疫炎症反应参与了疾病的病理过程。细胞因子在免疫应答和炎症反应中发挥重要作用，趋化因子是细胞因子家族中的重要成员。CXCL16是一种新型的趋化因子，通过其受体CXCR6参与炎性细胞向病变组织的趋化。本实验通过免疫组织化学法检测趋化因子CXCL16及其受体CXCR6在中重度牙周炎龈组织和健康牙龈组织中的表达情况及其相互关系。方法：收集辽宁省人民医院口腔科门诊就诊和住院患者的牙龈组织，年龄控制在35～74周岁，将其分为两组：健康牙龈组（40例）、中重度牙周炎组（39例）。健康牙龈组纳入标准：牙齿清洁，无龋洞，无疼痛感；牙龈颜色正常，无自发性或探诊出血。中重度牙周炎组入选标准参照第四版《牙周病学》。排除标准：有其他全身系统性疾病及药物过敏史；3个月内曾使用抗生素、肾上腺皮质激素、非甾体类抗炎药、性激素及避孕药等；6个月内有心梗发生；吸烟史。按照免疫组织化学染色法制片，光学生物显微镜下观察牙龈组织中CXCL16和CXCR6的分布和细胞定位，用免疫组织化学半定量分析(传统人工计数)法对数据进行综合评估，并使用SPSS15.0软件包对数据进行统计学分析。结果：1. CXCL16及CXCR6在中重度牙周炎患者的牙龈组织中均有表达。CXCL16主要表达在结缔组织区牙龈成纤维细胞、淋巴细胞（浆细胞）和血管内皮细胞的细胞膜及细胞质中，染色呈淡黄色至棕褐色；CXCR6主要表达在结缔组织区牙龈成纤维细胞、淋巴细胞（浆细胞）和血管内皮细胞的细胞膜上，染色呈淡黄色至棕褐色。 2. CXCL16在健康牙龈组织中无染色或散在淡黄色颗粒状染色，CXCR6在正常牙龈组织中未见或偶见淡黄色颗粒状染色。 3. CXCLl6在牙周炎龈组织、健康牙龈组织中的阳性表达率分别是92.31%、40.00%，，在牙周炎中以阳性表达为主，在健康牙龈组织中则低表达或无表达。中重度牙周炎组与健康牙龈组比较，CXCL16表达显著性升高，差异具有统计学意义(P0.05)。 4. CXCR6在牙周炎龈组织、健康牙龈组织中的阳性表达率分别是100%、30.00%；中重度牙周炎组与健康牙龈组比较，CXCR6表达显著性升高，差异具有统计学意义(P0.05)。 5．经相关性分析，CXCLl6与CXCR6在中重度牙周炎患者牙龈组织中的表达呈正相关(r=0.580，P0.05)，有统计学意义；而在健康牙龈组织中，二者的表达不相关。结论：1.趋化因子CXCLl6及其受体CXCR6在中重度牙周炎组中的表达高于健康牙龈组，提示CXCLl6、CXCR6可能与牙周炎的发病有关。 2．CXCLl6和CXCR6在中重度牙周炎龈组织中的表达呈正相关，提示二者在牙周炎的发病过程中可能共同发挥作用。
[Abstract]:Objective : To investigate the expression of chemokine CXCL16 and its receptor on the gingival tissues and healthy gingival tissues of moderate and severe periodontitis by immunohistochemical method . CXCL16 plays an important role in immune response and inflammatory response .

Methods : The gingival tissues of the outpatient department and the hospitalized patients were collected from the Department of Stomatology of the People ' s Hospital of Liaoning Province . The age was controlled between 35 and 74 years , and the patients were divided into two groups : healthy gum group ( 40 cases ) and moderate and severe periodontitis group ( 39 cases ) .

Healthy gingival group included standard : tooth cleaning , no caries hole , no pain feeling ;
The gingival color is normal without spontaneous or diagnostic bleeding . The criteria for inclusion in the moderate and severe periodontitis group are referred to in the 4th edition of the periodontal disease group .

Exclusion criteria : there are other systemic diseases and drug allergies ;
antibiotics , adrenocorticoids , non - steroidal anti - inflammatory drugs , sex hormones and contraceptives have been used within 3 months ;
myocardial infarction occurred within 6 months ;
History of smoking .

According to the immunohistochemical staining method , the distribution and cell localization of CXCL16 and CXCR4 6 in gingival tissues were observed under the optical biological microscope . The data were comprehensively evaluated by semi - quantitative analysis ( traditional manual counting ) method , and the data were analyzed statistically by SPSS 15.0 .

Results : 1 . CXCL16 was expressed in gingival fibroblasts , lymphocytes ( plasma cells ) and vascular endothelial cells of connective tissue . CXCL16 was mainly expressed in gingival fibroblasts , lymphocytes ( plasma cells ) and vascular endothelial cells .
On the cell membranes of gingival fibroblasts , lymphocytes ( plasma cells ) and vascular endothelial cells in connective tissue , the staining was pale yellow to tan .

2 . The CXCL16 did not stain or disperse in pale yellow granular staining in healthy gum tissue .

3 . The positive expression rates of CXCLl6 in gingival tissues and healthy gums were 92.31 % and 40.00 % , respectively .

4 . The positive expression rates were 100 % and 30.00 % in the gingival tissues of periodontitis and healthy gum tissues , respectively .
Compared with the healthy gum group in moderate and severe periodontitis group , there was a statistically significant difference in the expression of IL 6 in the patients with moderate and severe periodontitis ( P0.05 ) .

5 . The correlation analysis showed that CXCLl6 had positive correlation between CXCLl6 ( r = 0.580 and P0.05 ) in the gingival tissues of patients with moderate and severe periodontitis ( r = 0.580 , P0.05 ) .
In healthy gum tissue , the expression of the two is not related .

Conclusion : 1 . The expression of chemokine CXCLl6 and its receptor in moderate and severe periodontitis group is higher than that of healthy gum group , and suggests CXCLl6 and CXCLl6 may be related to the pathogenesis of periodontitis .

2 . The expression of CXCLl6 and CD6 in gingival tissues of moderate and severe periodontitis was positively correlated , suggesting that both might play a role in the pathogenesis of periodontitis .

【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R781.42

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