白细胞介素-17对人牙周膜成纤维细胞表达RANKL、OPG的影响

发布时间：2018-03-26 13:50

本文选题：牙周膜　切入点：成纤维细胞　出处：《重庆医学》2017年23期

【摘要】：目的研究白细胞介素17(IL-17)水平对人牙周膜成纤维细胞(HPDLF)中细胞核因子κB受体活化因子配体(RANKL)、骨保护素(OPG)表达的影响,探讨IL-17与正畸牙根吸收的相关性。方法采用组织块培养法,在体外建立人牙周膜成纤维细胞系。以不同水平(0、5、10、20、40ng/mL)的IL-17作用HPDLF 24h。采用RT-PCR和ELISA法,分别检测RANKL和OPG的mRNA、蛋白的表达。结果 (1)在0ng/mL组中,HPDLF表达RANKL、OPG。(2)0~20ng/mL各组中,HPDLF中RANKL的mRNA、蛋白表达量与IL-17水平呈正相关性。(3)5~20ng/mL各组中,OPG的mRNA、蛋白表达量与IL-17水平呈负相关性。(4)0~20ng/mL组中,RANKL/OPG的mRNA、蛋白的比值与IL-17水平呈正相关性。结论在无IL-17刺激时,HPDLF可表达RANKL、OPG。IL-17能够促进HPDLF表达RANKL,同时抑制HPDLF表达OPG,上调RANKL/OPG的比值。
[Abstract]:Objective to study the effect of interleukin 17 (IL 17) on the expression of nuclear factor 魏 B receptor activating factor ligand RANKL L (osteoprotegerin) in human periodontal ligament fibroblasts (HPD LF), and to explore the relationship between IL-17 and orthodontic root resorption. A human periodontal ligament fibroblast cell line was established in vitro. The HPDLF was treated with IL-17 at different levels (51010ng / mL) for 24 h. RT-PCR and ELISA methods were used. MRNAs and protein expressions of RANKL and OPG were detected respectively in 0ng/mL group. Results 1) in 0ng/mL group, the expression of RANKL in IL-17 was positively correlated with IL-17 level, and there was a negative correlation between protein expression and IL-17 level. The ratio of mRNA and protein of RANKL / OPG in 20 ng / mL group was positively correlated with the level of IL-17. Conclusion in the absence of IL-17 stimulation, the expression of RANKL / OPG.IL-17 can promote the expression of RANKL, inhibit the expression of HPDLF and up-regulate the ratio of RANKL/OPG.
【作者单位】：广西医科大学附属口腔医院正畸科;
【基金】：国家自然科学基金资助项目(81360170)
【分类号】：R783.5

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