不同时机转染基因对下颌骨牵引区细胞周期蛋白表达的影响研究

发布时间：2018-04-06 02:39

本文选题：转染时机　切入点：电穿孔　出处：《重庆医学》2017年20期

【摘要】：目的通过观察不同时机转染基因对下颌骨牵引区细胞周期蛋白表达的影响,探讨基因治疗促进牵引成骨的分子机制及最佳时机。方法 48只新西兰大白兔建立双侧下颌骨牵引成骨模型后均分为A、B、C、D组。A、B、C组分别于术后即刻、术后第4天、术后第14天在牵引区转染重组质粒pIRES-hBMP2-hVEGF165,并局部给予电穿孔刺激。4组动物均在术后第4天,以1mm/d的速度持续牵引10d后进入固定期。于固定期第7、14、28、56天各组分别处死动物3只,通过免疫组织化学染色检查牵引区新生骨组织cyclin A、D1、E的表达。结果在固定期第7、14天时,A、B、C组牵引间隙cyclin A、D1、E的表达较强,在第7天时表达最强烈,并且A、B、C组均较D组表达强;第28、56天时各组表达均较弱。在第7天时,B组表达较A、C、D组强(P0.05),A、C组间表达差异无统计学意义(P0.05),但均较D组强(P0.05);在第14天时,B、C组表达较A、D组强(P0.05),B、C组间及A、D组间表达差异无统计学意义(P0.05)。结论 cyclin A、D1、E的高表达能促进牵引区细胞分裂、增殖和分化,进而加快牵引区新骨形成,牵引期是基因治疗干预下颌骨牵引成骨的最佳时机。
[Abstract]:Objective to investigate the molecular mechanism and optimal time of gene therapy for promoting distraction osteogenesis by observing the effect of gene transfection on the expression of cyclin in mandibular traction region.Methods Forty-eight New Zealand white rabbits were divided into two groups after bilateral mandibular distraction osteogenesis.The recombinant plasmid pIRES-hBMP2-hVEGF165was transfected into the traction region on the 14th day after operation, and the animals in the group of local electroporation were treated with electroporation stimulation on the 4th day after operation. The animals in the control group were treated with 1mm/d at the rate of 1mm/d for 10 days and then entered the fixed period.Three animals were killed in each group on day 7, 14, 28 and 56 of the fixed period, and the expression of cyclin Agna D _ (1) E was detected by immunohistochemical staining in the nascent bone tissue of the traction area.Results the expression of cyclin in the tractive space was stronger in group A than in group C on day 7 and day 7, and the expression in group C was stronger than that in group D on day 7, and the expression was weaker in group C on day 2856.Conclusion the high expression of cyclin Agna D1OE can promote the cell division, proliferation and differentiation in the traction area, and then accelerate the formation of new bone in the traction area. The distraction stage is the best time for gene therapy to interfere with mandibular distraction osteogenesis.
【作者单位】：西南医科大学附属医院外科;济宁医学院附属医院烧伤整形科;南京医科大学附属友谊整形外科医院;
【基金】：国家自然科学基金资助项目(30600653) 四川省卫生厅科研课题(12226)
【分类号】：R782

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