人牙周膜干细胞成骨相诱导后相关基因的表达变化

发布时间：2018-04-06 03:20

本文选题：牙周膜干细胞　切入点：成骨诱导　出处：《上海口腔医学》2014年04期

【摘要】：目的:对人牙周膜干细胞进行筛选纯化,骨向诱导后观察其成骨能力,并对成骨相分化过程中相关基因的表达变化进行检测。方法:用组织块联合磁珠分选法分离纯化人牙周膜干细胞,初步探讨其成骨性能,碱性磷酸酶(ALP)和茜素红染色观察其成骨情况,实时定量PCR检测其相关成骨基因的表达变化。利用SPSS12.0软件包对数据进行统计学分析。结果:组织块联合磁珠分选法能成功培养出高纯度的人牙周膜干细胞。体外培养中,PDLSCs具备成脂和成骨的双相分化能力。在成骨诱导过程中,FOXO1和RUNX2的表达先升高再降低,ALP和OCN的基因表达水平持续增高。结论:成骨相关基因ALP、RUNX2、FOXO1和OCN均参与其向成骨样组织分化的过程,其表达变化具有时间规律性,与成骨过程类似。
[Abstract]:Objective: in human periodontal ligament stem cells were purified to observe the bone after induction of osteogenesis, and osteogenic differentiation phase expression of relevant genes in the process of testing. Methods: isolation and purification of human periodontal ligament stem cells with tissue combined with Macs, to explore its osteogenic properties. Alkaline phosphatase (ALP) and alizarin red staining to observe the osteogenesis, real-time quantitative PCR to detect the expression of genes related to bone. Packet data were analyzed by SPSS12.0 software. Results: the tissue block combined with Macs can successfully produce high purity human periodontal ligament stem cells. In vitro. PDLSCs with the adipogenic and osteogenic differentiation ability in dual phase. During osteogenic induction, the expression of FOXO1 and RUNX2 increased first and then decreased, ALP and OCN gene expression level continues to increase. Conclusion: the osteogenic related genes ALP, RUNX2, FOXO1 and OCN are involved in In the process of osteogenic tissue differentiation, its expression changes have time regularity, which is similar to the process of osteogenesis.

【作者单位】：上海交通大学医学院附属第九人民医院·口腔医学院口腔颅颌面科上海市口腔医学重点实验室;
【基金】：国家自然科学基金(81371178) 上海市科学技术委员会基础研究重点项目(12JC1405700) 上海交通大学医工交叉基金(YG2012MS40) 上海交通大学SMC-晨星青年学者奖励计划优秀青年教师(B类计划) 上海市教育委员会创新团队~~
【分类号】：R780.2

【相似文献】