三种药物预防和治疗早期牙酸蚀症效果的比较研究

发布时间：2018-04-15 18:18

本文选题：酸蚀症 + 奥威尔　；参考：《华北理工大学》2015年硕士论文

【摘要】：目的本实验主要通过离体牙的体外实验,观察奥威尔牙齿脱敏剂、多乐氟、碳酸氢钠矿化液对早期釉质酸蚀症再矿化及抗酸能力的影响,从而探索出一种再矿化及抗酸效果均较好的制剂,为临床上预防和治疗早期牙釉质脱矿提供有力的实验依据。方法选择华北理工大学博创口腔医院颌面外科拔除的无龋后牙60颗,制备成4mm×4mm的釉质样本120个,随机分为8组,每组15个。①再矿化实验:釉质标本经可口可乐浸泡脱矿后再用奥威尔牙齿脱敏剂(A组)、多乐氟(B组)、碳酸氢钠矿化液(C组)、去离子水对照组(D组)局部处理7天;②抗酸实验:经奥威尔牙齿脱敏剂(E组)、多乐氟(F组)、碳酸氢钠矿化液(G组)、去离子水对照组(H组)局部处理7天后,再经可口可乐浸泡脱矿。两组实验均应用扫描电镜、原子力显微镜、X射线能谱仪、显微硬度计进行检测,从釉质表面形态、显微结构、粗糙度、无机物含量和显微硬度等方面进行结果分析,评估三种药物对釉质的再矿化和抗酸能力。③采用SPSS17.0单因素方差分析对各组数据进行两两比较。结果1.扫描电镜观察结果:再矿化实验后,A、B、C各组均有矿物质沉积于釉质表面凹陷内,D组无明显变化;抗酸实验后,E、F、G、H各组釉质表面出现不同程度的破坏,H组脱矿凹陷明显。2.原子力显微镜观测结果:再矿化实验后,A、B、C组釉质表面呈现不同形态矿物质沉积,A组沉积物较另两组更规则明显,但D组仍呈典型釉质脱矿表现,A组凹陷深度显著小于B、C、D组(P0.05),A、B组粗糙度显著小于C、D组(P0.05),但A、B组间无明显差异(P0.05);抗酸实验后,E、F、G、H各组釉质表面出现不同程度的脱矿凹陷,E组凹陷明显浅于其他三组(P0.05),E、F组粗糙度明显小于G、H组(P0.05),但E、F组间无明显差异(P0.05)。3.能谱分析结果:再矿化实验后,A组的Ca2+、P3+含量都高于其他实验组(P0.05),但各组间Ca/P无明显差异(P0.05);抗酸实验后,E组的Ca2+、P3+含量高于其它实验组(P0.05),F组Ca/P明显低于E组(P0.05),而其他三组见未见明显差异(P0.05)。4.釉质表面显微硬度变化:再矿化实验和抗酸实验后,奥威尔牙齿脱敏剂组(A、E组)的显微硬度均优于实验中其他各组(P0.05)。结论1奥威尔牙齿脱敏剂、多乐氟和碳酸氢钠矿化液三种药物均可促进釉质再矿化、提高釉质抗酸能力。2经奥威尔牙齿脱敏剂、多乐氟处理后的釉质表面粗糙度相似。3奥威尔牙齿脱敏剂在促进釉质再矿化和提高釉质抗酸能力的方面均优于多乐氟和碳酸氢钠再矿化液。
[Abstract]:Objective to observe the effects of Orwell's tooth desensitizer, dolafur and sodium bicarbonate on the remineralization and anti-acid ability of early enamel acid etching.Thus, a new preparation with good effect of remineralization and acid resistance was developed, which provides a powerful experimental basis for clinical prevention and treatment of early enamel demineralization.Methods A total of 60 caries-free posterior teeth were extracted from maxillofacial surgery in Bochuang Oral Hospital of North China University of Science and Technology. 120 enamel samples of 4mm 脳 4mm were prepared and randomly divided into 8 groups.The enamel samples were treated with Orwell tooth desensitizer group A, Dorafur group B, sodium bicarbonate group C, deionized water group D) for 7 days.2 Anti-acid experiment: after 7 days of local treatment with Orwell's tooth desensitizer group E, Dorafur group F, sodium bicarbonate group G, deionized water control group (H group) for 7 days, then decalcified with Coca Cola.Scanning electron microscope, atomic force microscope (AFM) X-ray spectrometer and microhardness meter were used to test the results of the two groups of experiments. The results were analyzed from the aspects of enamel surface morphology, microstructure, roughness, inorganic content and microhardness.To evaluate the remineralization and acid resistance of three drugs to enamel. 3 the data of each group were compared by SPSS17.0 univariate ANOVA.Result 1.The results of scanning electron microscope observation showed that there was no obvious change of mineral deposit in group D in enamel surface depression after remineralization test, but there was different degree of destruction on enamel surface in each group after acid resistance test. The demineralization depression of group H was obvious.Atomic force microscope (AFM) observation: after the remineralization experiment, the enamel surface of group A was more regular than that of the other two groups, and the surface of enamel in group A was more regular than that in group A with different forms of mineral deposits.However, typical demineralization of enamel in group D showed that the depth of depression in group A was significantly lower than that in group B (P 0.05), but the roughness of group B was significantly lower than that of group C (D), but there was no significant difference between group A and B (P 0.05A).The roughness of E group was obviously lower than that of other three groups. The roughness of group F was obviously lower than that of group G H, but there was no significant difference between group E and group F (P 0.05 路3).The results of energy spectrum analysis showed that the content of Ca2 P 3 in group A was higher than that in other experimental groups, but there was no significant difference in Ca/P between groups, and the content of Ca2 P 3 3 in group E was significantly higher than that in group F (P 0.05), but the content of Ca/P in group E was significantly lower than that in group E (P 0.05).In the other three groups, there was no significant difference (P 0.05. 4).The changes of surface microhardness of enamel: the microhardness of Orwell's tooth desensitizer group was better than that of other groups (P 0.05) after remineralization test and acid resistance test.Conclusion (1) Orwell's tooth desensitizer, dolafur and sodium bicarbonate can promote enamel remineralization and improve enamel's acid-resistant ability by orwell's tooth desensitizer.The surface roughness of enamel treated with dollofluoride was similar. 3. 3. Orwell tooth desensitizer was superior to Dorafur and sodium bicarbonate in promoting enamel remineralization and improving enamel acid resistance.
【学位授予单位】：华北理工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R781.1

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