牙髓干细胞在壳聚糖-纤维蛋白原复合支架材料上的黏附与增殖

发布时间：2018-04-20 03:21

本文选题：壳聚糖 + 纤维蛋白原　；参考：《中国组织工程研究》2017年10期

【摘要】：背景:随着组织工程技术的飞速发展,单一的生物支架材料很难满足组织工程的需要,通过合适的方法将两种或多种类型的材料组合起来,可以得到性能更加优良的复合支架材料。目的:检测牙髓干细胞在壳聚糖-纤维蛋白原复合支架材料上的黏附与增殖情况。方法:(1)采用改良酶消化法分离提取C57乳鼠牙髓干细胞,传代培养至第3代,体外成脂成骨诱导分化对细胞进行鉴定;(2)制备壳聚糖-纤维蛋白原复合支架材料,测定其孔径及孔隙率;(3)将壳聚糖-纤维蛋白原复合支架材料与第3代小鼠牙髓干细胞共培养,以细胞直接接种于培养板底部作为对照组,MTT法检测细胞增殖情况;(4)扫描电镜观察壳聚糖-纤维蛋白原复合支架材料的形态,细胞黏附、增殖及基质分泌情况。结果与结论:(1)成功分离培养C57小鼠牙髓干细胞,经成脂成骨诱导分化后结果呈阳性;(2)壳聚糖-纤维蛋白原复合支架材料孔径为(105.32±22.10)μm,孔隙率为(87.714±1.276)%;(3)牙髓干细胞在壳聚糖-纤维蛋白原复合支架材料上的增殖曲线与对照组基本一致,均为"S"型,培养第4-8天实验组增殖速度显著快于对照组,差异有显著性意义(P0.05);(4)共培养第2天,细胞与壳聚糖-纤维蛋白原复合支架材料黏附紧密,生长状态良好,第4天,细胞明显开始增殖,体积增大,有丰富的细胞外基质,第6天,铺满于整个材料表面及空隙;(5)壳聚糖-纤维蛋白原复合材料是一种适宜牙髓干细胞黏附与增殖的支架材料。
[Abstract]:Background: with the rapid development of tissue engineering technology, it is difficult for a single biological scaffold material to meet the needs of tissue engineering. More excellent composite scaffold materials can be obtained. Aim: to investigate the adhesion and proliferation of dental pulp stem cells on chitosan-fibrinogen composite scaffolds. Methods the dental pulp stem cells of C57 rat were isolated by modified enzyme digestion method and cultured to the third generation. The cells were identified by adipogenic osteogenesis induction differentiation in vitro. The chitosan fibrinogen composite scaffold was prepared. The chitosan / fibrinogen composite scaffold was co-cultured with the third generation mouse dental pulp stem cells by measuring its pore size and porosity. Cell proliferation was detected by MTT method. The morphology, cell adhesion, proliferation and matrix secretion of chitosan fibrinogen composite scaffold were observed by SEM. Results and conclusion: C57 mouse dental pulp stem cells were isolated and cultured successfully. The results of adipogenic osteogenesis induced differentiation were positive. The pore size of chitosan / fibrinogen composite scaffold was 105.32 卤22.10 渭 m, the porosity was 87.714 卤1.276%. The proliferation curve of dental pulp stem cells on chitosan fibrinogen composite scaffold was basically the same as that of control group. The proliferation rate of the experimental group was significantly faster than that of the control group on the 4-8 days after culture, and the difference was significant (P 0.05). On the second day of co-culture, the cells adhered closely to the chitosan / fibrinogen composite scaffold material, and the growth state was good, and on the 4th day, the growth rate of the experimental group was better than that of the control group. On the 6th day, chitosan / fibrinogen composite was a suitable scaffold for the adhesion and proliferation of dental pulp stem cells.
【作者单位】：新疆医科大学第一附属医院儿童口腔科-口腔预防科;四川大学华西口腔医院儿童口腔科;
【基金】：国家自然科学基金(81560178)~~
【分类号】：R783.1

【相似文献】