Biodentine的渗透性能及其对牙本质抗折性能影响的实验研究

发布时间：2018-04-29 13:04

本文选题：Biodentine + MTA　；参考：《南方医科大学》2017年硕士论文

【摘要】：研究背景Biodentine是基于MTA改良成的牙本质修复材料。Biodentine具有良好的封闭性能,可促进组织修复再生。研究表明,Biodentine还可作为充填物形成HA,渗透入牙本质小管,与牙本质接触形成机械固位,减少微渗漏,防止再感染。但Biodentine作为充填物在小管中的渗透程度及对牙本质抗折影响的研究较少。根管充填整体化(monoblock)代表着根管治疗新趋势,monoblock强调充填的封闭性及材料的流动性使其渗入小管形成微机械固位,增强牙根抗折性。而作为充填物,在实现充填整体化中起重要作用。综上,Biodentine可能会影响牙本质抗折性。本实验拟比较探究Biodentine与MTA存在于根管内的渗透程度及其对牙本质抗折性的影响。研究目的本实验拟建立Biodentine与MTA充填于根管内的体外模型,比较材料在牙本质小管中渗透程度及牙本质抗折性。材料与方法第一部分Biodentine渗透入牙本质小管中的扫描电镜观察收集离体牙45颗,分成三组:Biodentine组;MTA组和Vitapex组,每组15颗。充填物充填根管。三个月后取标本距根尖1mm与5mm处横截面。电镜下记录材料渗透深度,分析结果。第二部分Biodentine在牙本质小管中渗透深度最大值及渗透面积百分比的研究收集60颗离体牙,分成四组:对照组;Biodentine组;MTA组和Vitapex组。药物荧光标记后充填根管。三个月后取标本距根尖lmm与5mm处组织,LSCM下观察。计算药物在1mm与5mm处的渗透深度最大值和渗透面积百分比,分析结果。第三部分Biodentine对牙本质三点弯曲强度及弯曲模量影响的初步探究收集尖牙48颗,根中部分牙本质作为标本。符合标准的标本共40个,保存备用。标本分为5组,每组8颗。A组为阳性对照组,不作处理;余四组均拔髓。其中B组不做充填;C、D、E组分别充填Biodentine、MTA、Vitapex。标本保存三个月后行力学测试。结果1.距根尖1mm与5mm处各材料渗透深度最大值均值差异具显著统计意义:距根尖5mm处各材料渗透深度最大值均值明显大于lmm处且渗透分布更均匀;Biodentine和MTA在距根尖5mm处渗透深度最大值差异无统计意义。2.距根尖5mm处各材料渗透面积百分比均值明显大于1mm处;距根尖1mm处各材料渗透面积百分比差异无统计意义;距根尖5mm处Biodentine与MTA渗透面积百分比均值分别78.8%、72.3%,差异具统计学意义。3.A组分别与B、D、E组间标本三点弯曲强度差异具统计意义;C组与D组相比,标本三点弯曲强度差异无统计意义。4.标本弯曲模量差异除了C组与D组无统计意义外,其他组间差异均具统计意义。结论1.成功建立Biodentine与MTA充填于根管内的体外模型。2.三种材料微粒密集附着于牙本质小管中及管周表面,小管未见破坏腐蚀。但Biodentine、MTA部分微粒渗入胶原纤维间,且Vitapex渗透深度明显不及Biodentine 与 MTA。3.距根尖5mm处各材料渗透深度最大值均值与渗透面积百分比均值明显大于 1mm处。4.Biodentine和MTA对牙本质抗折性的影响无统计差异。
[Abstract]:Background Biodentine is a modified dentin repair material based on MTA. Biodentine has good sealing performance and can promote tissue regeneration. The results showed that Biodentine could also be used as a filling material to form HA, infiltrate into dentin tubules, form mechanical retainer in contact with dentin, reduce microleakage and prevent re-infection. However, there are few studies on the permeability of Biodentine in the tubules and the effect of Biodentine on dentin fracture. Monoblock represents a new trend of root canal therapy. It emphasizes the sealing of filling and the fluidity of materials, which makes it infiltrate into the tubules to form micromechanical retainers and enhance the root fracture resistance. As a filling material, it plays an important role in the realization of filling integration. Biodentine may affect dentin fracture resistance. The purpose of this study was to compare the osmotic degree of Biodentine and MTA in root canal and its effect on dentin fracture. Objective to establish an in vitro model of Biodentine and MTA filling in root canals and compare the osmotic degree and dentin fracture resistance of the materials in dentine tubules. Materials and methods in part one, 45 teeth were collected by scanning electron microscope (SEM) after Biodentine permeated into dentine tubules. 45 teeth were divided into three groups, one group was divided into three groups: Biodentine group (n = 15) and Vitapex group (n = 15). The filling fills the root canal. Three months later, the cross section between 1mm and 5mm was taken. The penetration depth of the material was recorded under electron microscope and the results were analyzed. In the second part, the maximum osmotic depth and the percentage of osmotic area in dentine tubules were collected and 60 teeth were divided into four groups: control group, Biodentine group and Vitapex group. The root canal was filled with drug fluorescence labeling. After 3 months, the specimens were observed under lmm and 5mm. The maximum osmotic depth and the percentage of osmotic area at 1mm and 5mm were calculated and the results were analyzed. The third part: the effect of Biodentine on the three-point bending strength and flexural modulus of dentin. 48 canine teeth were collected and some dentin in the root were taken as specimens. A total of 40 specimens that meet the standards are preserved and reserved. The specimens were divided into 5 groups, 8 in each group were positive control group, untreated, and the other four groups were pull-out. Group B was not filled with Biodentine MTAA Vitapex. After three months of preservation, the specimens were subjected to mechanical tests. Result 1. Significant statistical significance was found in the difference of the mean value of the maximum osmotic depth between 1mm and 5mm: the mean value of the maximum osmotic depth of each material from 5mm to the root tip was significantly larger than that from lmm, and the osmotic distribution was more uniform. Biodentine and MTA permeated at 5mm from the root tip. The difference of maximum penetration depth has no statistical significance. The percentage of osmotic area from 5mm to root tip was significantly higher than that from 1mm, and there was no statistical significance between the percentage of osmotic area from root tip 1mm and that from root tip 1mm. The average osmotic area of Biodentine and MTA from the root tip of 5mm was 78.80.The difference was statistically significant. 3.The difference of three-point bending strength between group A and group E was statistically significant. There was no significant difference in three-point bending strength between group C and group D. Except group C and group D, the difference of flexural modulus was statistically significant. Conclusion 1. The in vitro model of Biodentine and MTA filling in root canal was established successfully. The three kinds of material particles were attached to the dentine tubules and the surface around the tubules. The tubules were not damaged and corroded. However, some particles of Biodentine Vitapex infiltrated between collagen fibers, and the penetration depth of Vitapex was significantly lower than that of Biodentine and MTA. 3. The mean value of maximum osmotic depth and the percentage of osmotic area from 5mm to root tip were significantly higher than those from 1mm. 4. There was no statistical difference in the effect of Biodentine and MTA on dentin fracture.
【学位授予单位】：南方医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R781.05

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