两种重编程系统诱导人牙源性多潜能干细胞的对比研究

发布时间：2018-05-02 18:28

本文选题：多潜能干细胞 + 乳头　；参考：《重庆医学》2017年01期

【摘要】：目的对比研究两种人牙源性多潜能干细胞重编程体系的特点。方法分别利用STEMCCA慢病毒/饲养层和仙台病毒/基质胶两种重编程系统,将人根尖乳头干细胞重编程为iPS细胞,比较两种方法的诱导效率、诱导工作量、iPS细胞非整倍体核型比例、外源性基因消除难易程度等情况。结果STEMCCA重编程体系需要制备饲养层细胞MEF,重编程效率约为0.1%,后期经Cre-Loxp酶切技术可得到无外源性转录基因的iPS细胞。仙台病毒重编程体系为无饲养层培养,基质胶制备方便、标准统一,重编程效率约为0.7%,明显高于STEMCCA系统(P0.05),外源性病毒和转录基因经自然传代后逐渐消除。结论与STEMCCA系统相比,仙台病毒/基质胶体系更适于人牙源性iPS细胞的诱导。
[Abstract]:Objective to compare the characteristics of two human odontogenic pluripotent stem cell reprogramming systems. Methods using two reprogramming systems of STEMCCA lentivirus / feeder layer and Sendai virus / matrix gel, human root tip papillary stem cells were reprogrammed into iPS cells, and the induction efficiency of the two methods, the induced workload, and the ratio of the aneuploidy karyotype of iPS cells were compared. The STEMCCA reprogramming system needs to prepare the feeder layer cell MEF, the reprogramming efficiency is about 0.1%, and the iPS cells without exogenous gene can be obtained by Cre-Loxp enzyme cutting technique in the later period. The reprogramming system of Sendai virus reprogramming system is no feeder layer culture, the preparation of matrix glue is convenient, the standard is unified and recompiled. The process efficiency was about 0.7%, obviously higher than the STEMCCA system (P0.05). The exogenous virus and the transcriptional gene were gradually eliminated after the natural passage. Conclusion compared with the STEMCCA system, Sendai virus / matrix glue system is more suitable for the induction of human odontogenic iPS cells.

【作者单位】：云南省第一人民医院/昆明理工大学附属医院口腔内科;昆明医科大学第一附属医院心内科;
【基金】：国家自然科学基金资助项目(81360161) 云南省教育厅基金资助项目(2015Y153)
【分类号】：R781

【相似文献】