低糖基化E-cadherins对舌鳞癌细胞增殖和侵袭的影响

发布时间：2018-05-07 13:27

本文选题：糖基化 + E-cadherin　；参考：《上海口腔医学》2014年01期

【摘要】：目的:研究低糖基化E-cadherins对舌鳞癌细胞CAL 27增殖和侵袭的影响,并探讨E-cadherins低糖基化修饰对其介导的细胞间黏附连接(adherens junctions,AJs)稳定性的影响。方法:用编码有FLAG标签的低糖基化Ecadherins和野生型E-cadherins质粒分别转染CAL 27细胞。48 h后,用抗FLAG抗体作为一抗,Western免疫印迹检测外源性E-cadherins的表达;用细胞增殖计数、单层细胞划痕愈合和细胞体外侵袭实验检测细胞的增殖和侵袭能力;免疫沉淀法检测E-cadherins介导的AJs复合体中的α-catenins、β-catenins、γ-catenins和vinculins的含量。采用SPSS17.0软件包对数据进行统计学分析。结果:被转染细胞均有外源性E-cadherins表达。被转染细胞的增殖和侵袭能力比未被转染对照细胞显著减弱(P0.05),而且低糖基化E-cadherins对CAL 27细胞增殖和侵袭能力的抑制作用比野生型E-cadherins显著增强(P0.05)。低糖基化E-cadherins介导的AJs复合体中的α-catenins、β-catenins、γ-catenins和vinculins的含量均比野生型E-cadherins介导的AJs复合体显著增多(P0.01)。结论 :低糖基化Ecadherins比野生型E-cadherins更加显著抑制舌鳞癌细胞的增殖和侵袭,其机制是低糖基化E-cadherins介导的AJs比野生型E-cadherins介导的AJs更加稳定。
[Abstract]:Aim: to study the effect of low-glycosylation E-cadherins on the proliferation and invasion of tongue squamous carcinoma cell CAL 27, and to investigate the effect of low glycosylation modification of E-cadherins on the stability of intercellular adhesion junctions (AJs) mediated by E-cadherins. Methods: the low glycosylated Ecadherins and wild type E-cadherins plasmids encoding FLAG tags were transfected into CAL 27 cells for 48 h, then the expression of exogenous E-cadherins was detected by anti FLAG antibody as an anti Western blot, and the cell proliferation was counted. The proliferation and invasiveness of monolayer cells were detected by scratch healing and invasiveness assay in vitro, and the levels of 伪 -catenins, 尾 -catenins, 纬 -catenins and vinculins in AJs complexes mediated by E-cadherins were detected by immunoprecipitation. The data were analyzed by SPSS17.0 software package. Results: exogenous E-cadherins expression was found in all transfected cells. The proliferation and invasion ability of transfected cells was significantly lower than that of untransfected control cells, and the inhibitory effect of low-glycosylated E-cadherins on the proliferation and invasion of CAL 27 cells was significantly enhanced than that of wild type E-cadherins cells. The contents of 伪 -catenins, 尾 -catenins, 纬 -catenins and vinculins in AJs complexes mediated by low-glycosylated E-cadherins were significantly higher than those in wild-type E-cadherins mediated AJs complexes. Conclusion: low glycosylated Ecadherins can significantly inhibit the proliferation and invasion of tongue squamous carcinoma cells compared with wild type E-cadherins. The mechanism is that low glycosylated E-cadherins mediated AJs is more stable than wild type E-cadherins mediated AJs.
【作者单位】：山东大学口腔医学院;山东省口腔生物医学重点实验室;
【基金】：教育部第40批留学回国人员科研启动基金项目山东省优秀中青年科学家科研奖励基金(BS2010YY021)
【分类号】：R739.8

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