葡萄糖浓度对婴幼儿牙菌斑中白色念珠菌产酸和粘附性的影响

发布时间：2018-05-24 07:31

本文选题：婴幼儿龋 + 白色念珠菌(CA)　；参考：《实用口腔医学杂志》2014年01期

【摘要】：目的:探讨葡萄糖浓度对婴幼儿龋(early childhood caries,ECC)和无龋(caries-free,CF)儿童牙菌斑中白色念珠菌临床分离菌株的产酸性和粘附性的影响。方法:将白色念珠菌临床分离菌株(ECC组26株,CF组24株),接种至不同葡萄糖浓度的沙氏液体培养基,以pH计测定其产酸能力;同时将菌液接种至羟基磷灰石片表面,对粘附至羟基磷灰石片表面的白色念珠菌进行平板培养,采用菌落形成单位计数法评定其粘附能力。结果:ECC组和CF组白色念珠菌的产酸和粘附能力随培养基中葡萄糖浓度(0.01~0.15 mol/L)的升高而增强。但在葡萄糖浓度为0.1 mol/L与0.15 mol/L时,2组白念珠菌的产酸能力无统计学差异(P0.05)。在同一葡萄糖浓度下,ECC组和CF组中白色念珠菌的产酸和粘附能力的差异无统计学意义(P0.05)。结论:在一定浓度范围内,葡萄糖可增强白色念珠菌临床菌株的产酸及粘附能力;婴幼儿牙菌斑中白色念珠菌的产酸性和粘附性不存在菌株间的差异性。
[Abstract]:Objective: to investigate the effects of glucose concentration on the acidity and adhesion of Candida albicans clinical isolates from dental plaque of infants with early childhood caries (Ecc) and caries-free CFCs (Caries free). Methods: Twenty-six strains of Candida albicans in ECC group and 24 strains in CF group were inoculated into Salvia liquid medium with different glucose concentration, and their acid-producing ability was measured by pH, and the bacteria solution was inoculated on the surface of hydroxyapatite tablet. The adherence of Candida albicans to hydroxyapatite tablets was evaluated by colony forming unit count method. Results the acid production and adhesion of Candida albicans were increased with the increase of glucose concentration of 0. 01 ~ 0. 15 mol / L in culture medium. But when glucose concentration was 0. 1 mol/L and 0. 15 mol/L, there was no significant difference in acid production ability of Candida albicans between the two groups. Under the same glucose concentration, there was no significant difference in acid production and adhesion ability of Candida albicans between ECC group and CF group. Conclusion: glucose can enhance the acid production and adhesion ability of clinical strains of Candida albicans in a certain concentration range, but there is no difference between strains in acid production and adhesion of Candida albicans in dental plaque of infants.
【作者单位】：中山大学附属口腔医院儿童口腔科·广东省口腔医学重点实验室;江门市中心医院口腔科;
【基金】：广东省科技计划社会发展项目(编号:2009B030801183);广东省科技计划国际合作项目(编号:2010B050700004) 广东省医学科研基金(编号:B2010117) 中山大学实验教学研究(改革)项目(编号:YJ201043)
【分类号】：R781.1

【参考文献】