TLR4,NOD2和NLRP3在牙髓炎中的表达

发布时间：2018-06-04 00:30

  本文选题：TLR + NOD　； 参考：《口腔医学研究》2017年05期

【摘要】：目的:分析牙髓炎中TLR4、NOD2和NLRP3的表达特征。方法:收集健康、龋病及牙髓炎的患牙共47颗分为3个组,采用免疫组织化学染色和单色免疫荧光染色方法检测每组牙髓组织中TLR4、NOD2、NLRP3的表达。结果:健康组中TLR4、NOD2和NLRP3仅在成牙本质细胞中少量表达。TLR4、NOD2和NLRP3在牙髓炎组及龋病组表达均明显高于正常组,大量表达于排列紊乱的成牙本质细胞和多种炎症细胞中,而在炎性病灶周边的牙髓成纤维细胞中三者也有表达。结论:本研究表明TLR4、NOD2和NLRP3在炎性牙髓组织中表达同时增高,提示三者均与牙髓炎的进展相关。
[Abstract]:Objective: to analyze the expression of TLR4, NOD2 and NLRP3 in pulpitis. Methods: a total of 47 teeth from healthy, dental caries and pulpitis were divided into 3 groups. The expression of TLR4, NOD2 and NLRP3 in each group was detected by immunohistochemical staining and monochromatic immunofluorescence staining. Results: the expression of TLR4NOD2 and NLRP3 in odontoblast was significantly higher in the pulpitis group and caries group than in the normal group, and the expression of TLR4NOD2 and NLRP3 in the odontoblast cells was significantly higher than that in the normal group, and the expression of TLR4NOD2 and NLRP3 in odontoblast cells was significantly higher than that in the dental caries group. There were also three expressions in pulp fibroblasts around inflammatory lesions. Conclusion: the expression of TLR4, NOD2 and NLRP3 in inflammatory dental pulp tissue is increased simultaneously, suggesting that the expression of TLR4, NOD2 and NLRP3 are related to the progression of pulpitis.
【作者单位】： 武汉大学口腔医学院牙体牙髓科;武汉科技大学附属天佑医院口腔科;
【基金】：国家自然科学基金项目(编号:81271190)
【分类号】：R781.31
，

本文编号：1974965