葡萄糖调节蛋白78和基质金属蛋白酶在口腔鳞状细胞癌中的表达

发布时间：2018-06-04 01:13

本文选题：口腔鳞状细胞癌 + 葡萄糖调节蛋白　；参考：《中国医科大学学报》2014年09期

【摘要】：目的探讨葡萄糖调节蛋白78(GRP78)和基质金属蛋白酶(MMPs)在口腔鳞状细胞癌组织中的表达及意义,阐明GRP78在口腔鳞状细胞癌发生、发展中的作用。方法采用免疫组织化学方法检测26例口腔鳞状细胞癌组织和10例正常口腔组织中GRP78蛋白的表达;采用逆转录PCR检测正常口腔组织和口腔鳞状细胞癌组织中GRP78 m RNA的表达;采用免疫组织化学方法检测26例口腔鳞状细胞癌组织中MMP-2和MMP-9蛋白的表达。结果 GRP78蛋白在口腔鳞状细胞癌组织中的阳性表达率为100%(26/26),在正常口腔组织中的阳性表达率为20%(2/10),两者比较差异有统计学意义(P0.01);与高分化和中分化口腔鳞状细胞癌组织比较,低分化口腔鳞状细胞癌组织中的GRP78蛋白阳性表达强度明显增高(P0.05)。与正常口腔组织比较,GRP78 m RNA表达水平在口腔鳞状细胞癌组织中的表达增高(P0.05)。MMP-2和MMP-9蛋白在口腔鳞状细胞癌组织中的阳性表达率均为100%(26/26)。结论 GRP78和MMPs在口腔鳞状细胞癌组织中表达明显升高,提示口腔鳞状细胞癌中GRP78蛋白的表达可能与肿瘤的侵袭和转移相关。
[Abstract]:Objective to investigate the expression and significance of glucose regulated protein 78 (GRP78) and matrix metalloproteinase (MMPs) in oral squamous cell carcinoma (OSCC) and to elucidate the role of GRP78 in the development of oral squamous cell carcinoma (OSCC). Methods the expression of GRP78 protein was detected by immunohistochemistry in 26 cases of oral squamous cell carcinoma and 10 cases of normal oral tissue, and the expression of GRP78 m RNA in normal oral tissue and oral squamous cell carcinoma tissue was detected by reverse transcription PCR. The expression of MMP-2 and MMP-9 in 26 cases of oral squamous cell carcinoma (OSCC) was detected by immunohistochemical method. Results the positive expression rates of GRP78 protein in oral squamous cell carcinoma (OSCC) and normal oral tissues were 100 / 26 / 26 and 20 / 10 / 10 respectively, which were significantly different from those in well-differentiated and moderately differentiated oral squamous cell carcinoma (OSCC), and compared with those in well-differentiated and moderately differentiated oral squamous cell carcinoma (OSCC), the positive expression rate of GRP78 protein in oral squamous cell carcinoma (OSCC) was significantly higher than that in oral squamous cell carcinoma (OSCC). The positive expression of GRP78 protein in poorly differentiated oral squamous cell carcinoma (OSCC) was significantly increased (P 0.05). The expression of GRP78m RNA in oral squamous cell carcinoma (OSCC) was significantly higher than that in normal oral tissue. The positive expression rates of P0.05, MMP-2 and MMP-9 in oral squamous cell carcinoma (OSCC) were 100 / 26 / 26 / 26 respectively. Conclusion the expression of GRP78 and MMPs in oral squamous cell carcinoma is significantly higher than that in oral squamous cell carcinoma, suggesting that the expression of GRP78 protein may be related to the invasion and metastasis of oral squamous cell carcinoma.
【作者单位】：内蒙古民族大学第二临床医学院病理科;内蒙古民族大学分子病理学研究所;内蒙古林业总医院病理科;沈阳医学院病理生理学教研室;沈阳医学院颌面外科;沈阳医学院;
【基金】：国家自然科学基金(81260310) 辽宁省博士科研启动基金(20111126)
【分类号】：R739.8

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