不同采样方法对口腔变形链球菌检出率的影响

发布时间：2018-06-05 02:31

本文选题：采样方法 + 变形链球菌　；参考：《口腔医学研究》2017年10期

【摘要】：目的:比较浓缩含漱法、非刺激性唾液法和棉拭子法对高龋和健康人群口腔唾液中变形链球菌检出率的影响。方法:对60例高龋患者和60例无龋者分别用棉拭子法、非刺激性唾液法和浓缩含漱法进行取样,采用轻唾选择培养基对变形链球菌进行培养,结合显微镜观察对3种方法收集到的变形链球菌进行鉴定,并用SPSS 22.0对数据进行统计学分析。结果:3种采样方法在高龋者中检出率分别为93.33%、96.67%、91.67%,无龋者中检出率分别为56.57%、58.33%、48.33%,无统计学显著性差异。结论:3种方法口腔唾液取样一致性较好。其中,棉拭子法采集到的唾液具有良好的代表性。
[Abstract]:Objective: to compare the effects of concentrated gargle, non-irritating saliva and cotton swab on the detection rate of Streptococcus mutans in oral saliva of high caries and healthy population. Methods: 60 patients with high caries and 60 patients without caries were sampled by cotton swab method, non-irritating saliva method and concentrated gargle method, and Streptococcus mutans were cultured in light salivary selective medium. The Streptococcus mutans collected by three methods were identified by microscope, and the data were analyzed statistically by SPSS 22. 0. Results the detectable rates of the three sampling methods were 93.33 and 96.671.67 in the patients with high caries, and 56.57 in the patients without caries, and 48.33 in the patients without caries, respectively. There was no significant difference between the two groups. Conclusion the consistency of oral saliva sampling by the three methods is good. Among them, the saliva collected by cotton swab method has a good representativeness.
【作者单位】：兰州大学口腔医学院;
【基金】：甘肃省自然科学基金(编号:1308RJZA248) 兰州大学大学生创新创业行动计划项目(编号:20151073001456)
【分类号】：R781.1

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