TNF-α对SCAP与PDLSCs体外增殖活性影响的比较研究

发布时间：2018-07-04 16:14

本文选题：TNF-α + 根尖乳头干细胞　；参考：《新疆医科大学》2016年硕士论文

【摘要】：目的:体外培养鼠根尖乳头干细胞和牙周膜干细胞,比较研究肿瘤坏死因子α对两者增殖活性的影响,探讨年轻恒牙在炎症条件下,两者用于牙根再生的可能性。方法:取Wistar幼鼠根尖尚未发育完全的健康下颌牙,酶消化法获得根尖乳头干细胞和牙周膜干细胞,免疫荧光法鉴定两者干细胞表面标志物STRO-1的表达。选取第2代根尖乳头干细胞和牙周膜干细胞的单细胞悬液,采用MTT法比较两者的生长曲线,绘制生长曲线图。将含有肿瘤坏死因子α浓度为0、5、10、50ng/m L的培养基加入根尖乳头干细胞和牙周膜干细胞的第3代细胞中,使用MTT法检测两者各孔吸光密度值,比较其对两种细胞增殖活性的影响。结果:1.根尖乳头干细胞和牙周膜干细胞均呈典型的成纤维细胞样的梭形结构。2.根尖乳头干细胞和牙周膜干细胞均表达干细胞特性;3.根尖乳头干细胞和牙周膜干细胞各光孔密度值随时间的递增而呈现出增高的趋势。自第4天起根尖乳头干细胞的增殖活力明显强于牙周膜干细胞。4.在10ng/mL和50ng/mL浓度时,肿瘤坏死因子α能明显促进根尖乳头干细胞的增殖,亦能明显促进牙周膜干细胞的增殖。在10ng/mL和50ng/mL浓度时,根尖乳头干细胞的增殖能力强于牙周膜干细胞。结论:根尖乳头干细胞在正常状态和炎症状态下增殖活性均强于牙周膜干细胞,此研究结果为种子细胞在年轻恒牙发生根尖周炎症后牙根继续发育的能力提供实验依据。
[Abstract]:Objective: to culture rat apical papilla stem cells and periodontal ligament stem cells in vitro, to compare the effects of tumor necrosis factor 伪 on the proliferation of the two cells, and to explore the possibility of the young permanent teeth being used for root regeneration under the condition of inflammation. Methods: the apical papillary stem cells and periodontal ligament stem cells were obtained from immature healthy mandibular teeth of young Wistar rats. The expression of STRO-1 on the surface of stem cells was identified by immunofluorescence. Single cell suspensions of apical papilla stem cells and periodontal ligament stem cells of the second generation were selected and the growth curves of the two cells were compared by MTT method and the growth curves were plotted. The culture medium containing 50 ng / mL TNF- 伪 was added to the third passage cells of apical papillary stem cells and periodontal ligament stem cells. MTT assay was used to detect the absorptivity of the two cells and to compare their effects on the proliferative activity of the two kinds of cells. The result is 1: 1. The apical papilla stem cells and periodontal ligament stem cells were both typical fibroblast-like fusiform structures. Apical papilla stem cells and periodontal ligament stem cells both expressed stem cell characteristics. The optical pore density of apical papilla stem cells and periodontal ligament stem cells increased with time. The proliferative activity of apical papilla stem cells was significantly stronger than that of periodontal ligament stem cells. At the concentrations of 10 ng / mL and 50 ng / mL, TNF- 伪 significantly promoted the proliferation of apical papilla stem cells and periodontal ligament stem cells. At the concentrations of 10 ng / mL and 50 ng / mL, the proliferation of apical papillary stem cells was stronger than that of periodontal ligament stem cells. Conclusion: the proliferative activity of apical papillary stem cells is stronger than that of periodontal ligament stem cells in normal and inflammatory state. The results provide experimental evidence for the ability of seed cells to continue to develop after periapical inflammation in young permanent teeth.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R781.3

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