Farnesol作用变异链球菌脱矿的实验研究

发布时间：2018-07-28 13:26

【摘要】：目的 Farnesol(法尼醇)作为密度感应分子,具有影响变异链球菌生物膜代谢以及介导肿瘤细胞凋亡的作用。文中研究Farnesol对变异链球菌脱矿的影响。方法将变异链球菌UA159菌悬液接种于放置牙釉质切片的培养皿中,分为5组,即变链+0μmol/L Farnesol组、变链+50μmol/L Farnesol组、变链+100μmol/L Farnesol组、变链+200μmol/L Farnesol组和对照组。对照组中加入无菌去离子水。采用扫描电镜观察釉质表面的变异链球菌生物膜,显微硬度仪检测实验前后釉质表面显微硬度(surface microhardness,SMH),并用粗糙度仪测定釉面粗糙度。结果随着Farnesol浓度的递增,变异链球菌生物膜生长受抑制,釉质表面粗糙度减少,釉质显微硬度的下降幅度减小,当Farnesol浓度为200μmol/L时釉质显微硬度值[(270.87±12.19)N/mm2]较[变链+50μmol/L Farnesol组、变链+100μmol/L Farnesol组、变链+0μmol/L Farnesol组的(224.63±18.98)、(228.06±10.40)、(198.57±8.19)N/mm2]明显升高(P0.01),各实验组的SMH较对照组[(368.60±7.33)N/mm2]低,差异有统计学意义(P0.05)。结论 Farnesol可抑制变异链球菌对牙釉质的脱矿作用。
[Abstract]:Objective as a density sensing molecule, Farnesol can affect the biofilm metabolism of Streptococcus mutans and mediate the apoptosis of tumor cells. The effect of Farnesol on demineralization of Streptococcus mutans was studied. Methods the suspension of Streptococcus mutans (UA159) was inoculated into a petri dish with enamel sections. It was divided into 5 groups: 0 渭 mol/L Farnesol, 50 渭 mol/L Farnesol, 100 渭 mol/L Farnesol, 200 渭 mol/L Farnesol and control group. Aseptic deionized water was added to the control group. The enamel surface of Streptococcus mutans biofilm was observed by scanning electron microscope. The surface microhardness of enamel was measured by microhardness instrument before and after the experiment, and the roughness of enamel was measured by rough degree instrument. Results with the increase of Farnesol concentration, the growth of biofilm of Streptococcus mutans was inhibited, the surface roughness of enamel decreased, and the microhardness of enamel decreased. When the concentration of Farnesol was 200 渭 mol/L, the microhardness of enamel [(270.87 卤12.19) N/mm2] was higher than that of [50 渭 mol/L Farnesol] group. (224.63 卤18.98), (228.06 卤10.40), (198.57 卤8.19 N/mm2) in the 224.63 渭 mol/L Farnesol group and 0 渭 mol/L Farnesol group (P 0.01). The SMH in each experimental group was lower than that in the control group [(368.60 卤7.33) N/mm2] (P0.05). Conclusion Farnesol can inhibit the demineralization of Streptococcus mutans to enamel.
【作者单位】：南京医科大学口腔医学研究所;南京理工大学医院口腔科;
【基金】：国家自然科学基金(30872885)
【分类号】：R781.1

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