涎腺癌组织中EGFR和HER-2表达及临床意义

发布时间：2018-08-25 07:55

【摘要】：目的探讨黏液表皮样癌、腺样囊性癌和腺泡细胞癌中EGFR和HER-2基因/蛋白表达及临床意义。方法采用免疫组化EnVision两步法检测80例涎腺癌(黏液表皮样癌30例、腺样囊性癌30例、腺泡细胞癌20例)和30例良性多形性腺瘤中EGFR和HER-2蛋白表达;采用荧光原位杂交技术检测涎腺癌中EGFR和HER-2基因扩增。结果 (1)涎腺癌中EGFR和HER-2蛋白阳性率分别为71.25%和32.5%,高于良性多形性腺瘤组(P均0.05),其中以黏液表皮样癌中EGFR和HER-2蛋白阳性率最高,EGFR蛋白的表达强度最高(P均0.05)。(2)EGFR和HER-2蛋白表达与涎腺癌患者性别、年龄、肿瘤最大直径、肿瘤分化程度、组织学亚型无关(P均0.05);涎腺癌中EGFR和HER-2蛋白表达无相关性(rs=0.166,P0.05)。(3)涎腺癌中EGFR基因高多体扩增的总阳性率为18.75%(15/80),其中黏液表皮样癌13例,腺样囊性癌2例,阳性率分别为43.3%和6.7%,相应蛋白表达强度均为(嘌)或(嘁),表达强度和基因扩增之间呈明显正相关(rs=0.491,P0.01);涎腺癌中未检测到HER-2基因扩增及17号染色体多体。(4)EGFR蛋白(嘌/嘁)组和基因扩增组患者的生存时间比蛋白(-/+)组和无基因扩增组均明显缩短(P均0.05)。结论黏液表皮样癌高频率、高强度表达EGFR,并发生高多体基因扩增,可作为其分子靶向治疗的靶点。黏液表皮样癌、腺样囊性癌和腺泡细胞癌中HER-2蛋白弱表达,未检测到基因扩增。
[Abstract]:Objective to investigate the expression and clinical significance of EGFR and HER-2 gene / protein in mucoepidermoid carcinoma, adenoid cystic carcinoma and acinar cell carcinoma. Methods the expression of EGFR and HER-2 protein in 80 cases of salivary gland carcinoma (30 cases of mucoepidermoid carcinoma, 30 cases of adenoid cystic carcinoma, 20 cases of acinar cell carcinoma) and 30 cases of benign pleomorphic adenoma were detected by immunohistochemical EnVision two-step method. The amplification of EGFR and HER-2 genes in salivary gland carcinoma was detected by fluorescence in situ hybridization. Results (1) the positive rates of EGFR and HER-2 protein in salivary gland carcinoma were 71.25% and 32.5%, respectively, which were higher than those in benign pleomorphic adenoma group (P 0.05). The highest positive rate of EGFR and HER-2 protein in mucoepidermoid carcinoma was found in mucoepidermoid carcinoma (P 0.05). (2). Protein expression and sex in patients with salivary gland carcinoma, There was no correlation between EGFR and HER-2 protein expression (rs=0.166,P0.05). (3) in salivary gland carcinoma (rs=0.166,P0.05). (3). The total positive rate of high multibody amplification of EGFR gene in salivary gland carcinoma was 18.75% (15 / 80), including 13 cases of mucoepidermoid carcinoma. 2 cases of adenoid cystic carcinoma, The positive rates were 43. 3% and 6. 7%, respectively. The corresponding protein expression intensity was either puor or scratch, and there was a positive correlation between the expression intensity and gene amplification (rs=0.491,P0.01), no amplification of HER-2 gene and polyploid of chromosome 17 were detected in salivary gland carcinoma. (4) EGFR protein. The survival time of the patients in the scratch group and the gene amplification group was significantly shorter than that in the protein (- /) group and the non-gene amplification group (P 0.05). Conclusion the mucoepidermoid carcinoma has high frequency and high intensity of EGFR, expression and high polymorphic gene amplification. It can be used as a target for molecular targeting therapy of mucoepidermoid carcinoma. HER-2 protein was weakly expressed in mucoepidermoid carcinoma, adenoid cystic carcinoma and acinar cell carcinoma.
【作者单位】：江苏省连云港市第一人民医院病理科;江苏省连云港市第一人民医口腔科;
【基金】：连云港市卫生局课题(1206)
【分类号】：R739.87

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