刷牙对酸蚀后牙釉质表面磨损的观察
发布时间：2018-09-09 12:10
【摘要】：目的: 比较柠檬酸酸蚀后的牙釉质经过不同再矿化时间，刷牙对其表面磨损深度的影响，为口腔保健行为提供理论指导。 材料和方法: 1、经伦理委员会批准，选择10个志愿者，签署知情同意书。2、收集新鲜拔除、健康无龋的正畸减数的前磨牙80颗，制成5mm×5mm×2mm大小的牙釉质块，打磨，抛光后，用显微硬度计测量各个牙釉质的表面显微硬度，并挑选出60个显微硬度值最接近计算平均值的釉质块。3、为志愿者制作腭基托，随机分配釉质块，用蜂蜡固定在腭基托中，釉质近远中边缘分别用复合树脂覆盖2mm（釉质表面和基托边缘各1mm）。每个腭基托6个釉质块，分别为A-F组，每组10个样本。4、实验第一天：志愿者除进食时间和口腔清洁时间将腭基托取下，其余时间均需将腭基托置于口腔内，以便釉质块表面形成唾液获得性膜。实验第二至四天，志愿者每天进行两次刷牙实验（上午8：00和下午8:00），第四天下午刷牙实验结束后志愿者将不再需要佩戴腭基托，处理如下:首先用蜂蜡将F组釉质块覆盖，然后每个腭基托浸入50ml柠檬酸（pH3.2）中2分钟，最后将F组蜂蜡去除。A-D组进行不同的再矿化时间后进行刷牙（从腭基托边缘方向向中心方向统一力度磨刷40次）。A组：0分钟；B组：20分钟；C组：40分钟；D组：60分钟。E组：酸蚀并且再矿化，但不进行刷牙。F组：不酸蚀，再矿化，刷牙。5、实验结束后用轮廓仪测量各个牙釉质块的磨损深度，每个釉质块记录三个读数，取其平均值，六组间比较采用秩和检验的统计学方法；组间两两比较采用LSD方法；检验水准P0.05为差异有统计学意义。用电子扫描显微镜观察各组釉质块的表面形态。 结果: 1.牙釉质随着酸蚀后再矿化时间的增加，刷牙对其表面造成的磨损深度逐渐减小，组间差异具有统计学意义（P＜0.05）。 2.刷牙会对酸蚀后的牙釉质表面造成磨损，实验组A、B、C、D组釉质表面磨损深度与对照组E组比较，差异明显且有统计学意义（P＜0.05） 3.酸蚀对牙釉质表面磨损有影响，实验组A、B、C、D组与对照组F组比较，磨损深度增加，差异有统计学意义（P＜0.05） 4.牙釉质被酸蚀后再矿化时间越短，在电镜下显示其表面凹陷越大越深越多，表面形态越粗糙。 结论: 1.牙釉质被酸蚀后，立即刷牙会对牙釉质表面形成较大的磨损，，而随着酸蚀后牙釉质在唾液中的再矿化时间增加，刷牙对其造成的磨损深度减小。 2.进食酸性饮料后，即使使用含氟牙膏，间隔60分钟刷牙仍然对牙釉质有损害。
[Abstract]:Objective: to compare the effect of tooth brushing on the wear depth of enamel after citric acid etching after different remineralization time, and to provide theoretical guidance for oral health care. Materials and methods: 1. With the approval of the Ethics Committee, 10 volunteers were selected to sign the informed consent form. 80 premolars with fresh extraction and no caries were collected and made into enamel blocks of the size of 5mm 脳 5mm 脳 2mm, which were polished and polished. The surface microhardness of each tooth enamel was measured by microhardness meter, and 60 enamel blocks, which were closest to the calculated average value, were selected. The palatal base was made for volunteers, and the enamel mass was randomly allocated and fixed in the palatine base with beeswax. 2mm (enamel surface and base edge 1mm) were covered with composite resin at the distal edge of enamel. There were 6 enamel masses in each palatine base, each group with 10 samples. 4. The first day of the experiment: except for feeding time and oral cleaning time to remove the palatine base, the rest of the time should be placed in the mouth. In order to form a salivary acquired membrane on the enamel mass surface. From the second to the fourth day, the volunteers brushed their teeth twice a day (8:00 and 8:00). After the fourth day of brushing the teeth, the volunteers would no longer need to wear a palatine base. The treatment was as follows: first, the enamel of group F was covered with beeswax. Then each palatal base was immersed in 50ml citric acid (pH3.2) for 2 minutes, then the beeswax of group F was removed from beeswax for different remineralization time and then brushed (40 times from the palatine base edge to the center). Group A: 0 minutes; Group B: 20 minutes; group C: 40 minutes; group D: 60 minutes; group E: acid erosion and re-mineralization, but not brushing teeth. Group F: no acid erosion, remineralization, brushing teeth .5.After the experiment, the wear depth of each enamel mass was measured by profilometer. Each enamel block recorded three readings, take its average, six groups of comparison using rank sum test statistical method; two groups of comparison using LSD method; test level P0.05 for the difference was statistically significant. The surface morphology of each group of enamel was observed by electron scanning microscope. Results: 1. With the increase of remineralization time of enamel after etching, the wear depth caused by tooth brushing on its surface decreased gradually, and the difference between groups was statistically significant (P < 0.05). 2. Tooth brushing will cause wear on enamel surface after acid etching. The wear depth of enamel surface in group A BX CnD is significantly different from that in group E (P < 0.05). 3. The effect of brushing teeth on the surface of enamel in the experimental group was significantly higher than that in the control group (P < 0.05). Acid etching had an effect on the surface wear of enamel. Compared with the control group F, the wear depth of the experimental group was higher than that of the control group (P < 0.05). 4. The shorter the remineralization time of enamel is, the larger the surface depression is, the deeper the surface is, and the rougher the surface morphology is. Conclusion: 1. After the enamel was etched by acid, the enamel surface was worn by brushing immediately, but the wear depth decreased with the increase of the remineralization time of enamel in saliva. 2. Even if fluoride toothpaste is used, brushing teeth at intervals of 60 minutes will still damage enamel.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R780.1
本文编号：2232327
[Abstract]:Objective: to compare the effect of tooth brushing on the wear depth of enamel after citric acid etching after different remineralization time, and to provide theoretical guidance for oral health care. Materials and methods: 1. With the approval of the Ethics Committee, 10 volunteers were selected to sign the informed consent form. 80 premolars with fresh extraction and no caries were collected and made into enamel blocks of the size of 5mm 脳 5mm 脳 2mm, which were polished and polished. The surface microhardness of each tooth enamel was measured by microhardness meter, and 60 enamel blocks, which were closest to the calculated average value, were selected. The palatal base was made for volunteers, and the enamel mass was randomly allocated and fixed in the palatine base with beeswax. 2mm (enamel surface and base edge 1mm) were covered with composite resin at the distal edge of enamel. There were 6 enamel masses in each palatine base, each group with 10 samples. 4. The first day of the experiment: except for feeding time and oral cleaning time to remove the palatine base, the rest of the time should be placed in the mouth. In order to form a salivary acquired membrane on the enamel mass surface. From the second to the fourth day, the volunteers brushed their teeth twice a day (8:00 and 8:00). After the fourth day of brushing the teeth, the volunteers would no longer need to wear a palatine base. The treatment was as follows: first, the enamel of group F was covered with beeswax. Then each palatal base was immersed in 50ml citric acid (pH3.2) for 2 minutes, then the beeswax of group F was removed from beeswax for different remineralization time and then brushed (40 times from the palatine base edge to the center). Group A: 0 minutes; Group B: 20 minutes; group C: 40 minutes; group D: 60 minutes; group E: acid erosion and re-mineralization, but not brushing teeth. Group F: no acid erosion, remineralization, brushing teeth .5.After the experiment, the wear depth of each enamel mass was measured by profilometer. Each enamel block recorded three readings, take its average, six groups of comparison using rank sum test statistical method; two groups of comparison using LSD method; test level P0.05 for the difference was statistically significant. The surface morphology of each group of enamel was observed by electron scanning microscope. Results: 1. With the increase of remineralization time of enamel after etching, the wear depth caused by tooth brushing on its surface decreased gradually, and the difference between groups was statistically significant (P < 0.05). 2. Tooth brushing will cause wear on enamel surface after acid etching. The wear depth of enamel surface in group A BX CnD is significantly different from that in group E (P < 0.05). 3. The effect of brushing teeth on the surface of enamel in the experimental group was significantly higher than that in the control group (P < 0.05). Acid etching had an effect on the surface wear of enamel. Compared with the control group F, the wear depth of the experimental group was higher than that of the control group (P < 0.05). 4. The shorter the remineralization time of enamel is, the larger the surface depression is, the deeper the surface is, and the rougher the surface morphology is. Conclusion: 1. After the enamel was etched by acid, the enamel surface was worn by brushing immediately, but the wear depth decreased with the increase of the remineralization time of enamel in saliva. 2. Even if fluoride toothpaste is used, brushing teeth at intervals of 60 minutes will still damage enamel.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R780.1
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