重组人β防御素3对牙龈卟啉单胞菌脂多糖致炎作用的影响

发布时间：2018-09-09 16:58

【摘要】：目的:以牙龈卟啉单胞菌脂多糖(P.g-LPS)诱发载脂蛋白E基因敲除(ApoE-/-)小鼠和RAW264.7小鼠单核巨噬细胞的急性炎症反应,并应用重组人β防御素3(rhBD3),观察其对炎症的干预效果。方法:20周龄雄性ApoE-/-小鼠随机均分为PBS对照组、P.g-LPS组和P.g-LPS+rhBD3组,分别经腹腔注射给药2h后,检测血清中不同炎症指标(MCP-1、TNF-α、IL-6、IL-1β和NO)的水平。同时,以rhBD3干预P.g-LPS对RAW264.7细胞的致炎作用,分别检测培养上清和细胞中炎症指标的水平及其mRNA的相对表达量。结果:经P.g-LPS刺激后,ApoE-/-小鼠血清MCP-1、TNF-α、IL-6和NO的水平较对照组显著上调;而同时应用rhBD3能明显降低MCP-1、TNF-α和NO表达量。P.g-LPS能显著上调RAW264.7细胞培养上清中TNF-α和NO的水平以及细胞中TNF-α和IL-6的mRNA相对表达量,rhBD3对此具有抑制作用。结论:rhBD3对P.g-LPS在体内、外诱导的急性炎症具有一定的抑制效应,可能在牙周炎与高脂血症的相互作用中发挥免疫调节作用。
[Abstract]:Aim: to induce acute inflammatory response of macrophages from apolipoprotein E knockout (ApoE-/-) mice and RAW264.7 mice with lipopolysaccharide (P.g-LPS) from porphyromonas gingivalis, and to observe the effect of recombinant human 尾 -defensin 3 (rhBD3) on inflammation. Methods Twenty week-old male ApoE-/- mice were randomly divided into PBS control group and P.g-LPS rhBD3 group. The serum levels of different inflammatory markers (MCP-1,TNF- 伪 -IL-6, IL-1 尾 and NO) were measured after intraperitoneal injection for 2 hours. At the same time, rhBD3 was used to interfere with the inflammatory effect of P.g-LPS on RAW264.7 cells, and the levels of inflammatory markers and the relative expression of mRNA in the culture supernatants and cells were measured respectively. Results: the serum levels of MCP-1,TNF- 伪, IL-6 and NO in ApoE-r-mice stimulated by P.g-LPS were significantly higher than those in the control group. At the same time, rhBD3 significantly decreased the expression of MCP-1,TNF- 伪 and NO. P.g-LPS upregulated the levels of TNF- 伪 and NO in the supernatant of RAW264.7 cells, and the relative expression of TNF- 伪 and IL-6 in RAW264.7 cells was inhibited by rhBD3. Conclusion: rhBD3 has a certain inhibitory effect on acute inflammation induced by P.g-LPS in vivo and in vitro and may play an immunomodulatory role in the interaction between periodontitis and hyperlipidemia.
【作者单位】：南京大学医学院附属口腔医院牙周科;
【基金】：国家自然科学基金项目(编号:81371152) 江苏省自然科学基金项目(编号:BK20131079) 江苏省临床科技专项(编号:BL2013002)
【分类号】：R780.2

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