吴茱萸碱联合放射治疗对舌鳞癌细胞Tca-8113裸鼠移植瘤生长的抑制作用

发布时间：2018-11-27 07:09

【摘要】：目的:研究吴茱萸碱(evodiamine,EVO)联合射线对舌鳞癌细胞Tca-8113裸鼠移植瘤生长的抑制作用。方法:建立舌鳞癌Tca-8113细胞裸鼠皮下移植瘤模型16只,随机分为空白对照组、EVO组、放射组及联合组,观察并绘制肿瘤生长曲线图,计算肿瘤抑制率;肿瘤组织通过常规苏木精-伊红染色行病理学检查;免疫组织化学法检测移植瘤组织中Ki67、Bax和Bcl-2表达情况;透射电子显微镜观察细胞凋亡形态。结果:治疗第12天起,联合组肿瘤体积与其余各组相比,明显减小(P0.05);治疗结束时,EVO组、放射组和联合组的抑瘤率分别为15.15%、43.29%和63.20%。联合组Ki67和Bcl-2表达水平较EVO组和放射组下降,Bax表达水平增高(P0.05)。联合组坏死细胞数增多,且细胞呈典型凋亡特征。结论:放疗联合EVO能抑制舌鳞癌Tca-8113细胞裸鼠移植瘤的生长,可能与Ki67和Bcl-2表达下调以及Bax表达上调有关。
[Abstract]:Aim: to study the inhibitory effect of rutaecarpine (evodiamine,EVO) combined with radiation on the growth of tongue squamous cell Tca-8113 xenografts in nude mice. Methods: sixteen nude mice with tongue squamous cell carcinoma (Tca-8113) were randomly divided into three groups: blank control group, EVO group, radiation group and combined group. The tumor growth curve was observed and plotted to calculate the tumor inhibition rate. The expression of Ki67,Bax and Bcl-2 in transplanted tumor tissue was detected by immunohistochemical method and apoptosis morphology was observed by transmission electron microscope. Results: from the 12th day after treatment, the tumor volume of the combined group was significantly decreased compared with the other groups (P0.05), and at the end of the treatment, the tumor inhibition rates of the EVO group, the radiation group and the combined group were 15.15% and 63.20%, respectively. The expression of Ki67 and Bcl-2 in the combined group was lower than that in the EVO group and radiation group, and the Bax expression level was increased (P0.05). In the combined group, the number of necrotic cells increased and the cells showed typical apoptotic characteristics. Conclusion: radiotherapy combined with EVO can inhibit the growth of Tca-8113 xenografts in tongue squamous cell carcinoma, which may be related to the down-regulation of Ki67 and Bcl-2 and the up-regulation of Bax expression.
【作者单位】：兰州大学口腔医学院口腔内科;
【基金】：中央高校基本科研业务费专项资金资助自由探索面上项目(编号:lzujbky-2012-159) 甘肃省中医药科学技术研究课题(编号:GZK-2011-78) 兰州市科技计划项目(编号:2011-2-43)
【分类号】：R739.86

【共引文献】