骨膜牵张成骨术联合PRF扩增兔牙槽嵴的实验研究
发布时间：2018-11-27 07:26
【摘要】：目的:应用课题组专利牵张器,进行骨膜牵张成骨(periosteum distraction osteogenesis,PDO)联合富血小板纤维蛋白(platelet-rich fibrin,PRF)扩增兔下颌牙槽嵴,评价新骨生成情况。材料与方法:成年新西兰大白兔36只,随机分为A、B、C三组,每组12只。所有实验动物均拔除左侧下颌第一前磨牙,1个月后纳入实验。A组(PDO组):左侧拔牙区安置牵张器。B组(PDO+PRF):手术方法同A组,在牵引钛网表面放置PRF膜。C组(异种骨移植组):左侧拔牙区行5*5*5mm异种骨块移植术,表面生物膜覆盖。A、B组术后间歇7天,以0.25mm/次,2次/天的速度,连续牵张9天,固定第60天、120天分别处死6只动物;C组术后60天、120天分别处死6只动物。取材观察新骨生成情况:大体观察、新骨高度的测量、X线分析、Micro-CT检查、不脱钙硬组织切片观察、HE组织学观察、BMP-2免疫组化观察。结果:PDO组固定60天时,牵张区钛网下及牵引钉周围可见部分新骨形成,新骨平均高度为2.65±0.37mm;影像学显示,钛网下可见中、低密度新骨影像,结构疏松;硬组织切片及HE染色可见术区大量新生骨小梁,成骨活跃。固定120天时,钛网下新骨趋于成熟与自体骨融为一体,新生骨高度:4.13±0.31mm;影像学显示,钛网下新骨高密影,骨小梁结构清晰,与周围牙槽骨融为一体;组织学观察,新生骨趋于成熟,可见成熟哈弗系统,与自体牙槽骨相延续。B组上述观察结果与A组相类似,C组移植骨块呈现出逐渐吸收,被新生骨“爬行替代”的过程。结论:使用课题组专利牵张器牵张兔下颌牙槽嵴骨膜,固定60天、120天发现牵张间隙有新骨形成。PRF对PDO未发现明显促进作用。PDO操作简便、手术创伤小、无需植骨,有着很大的临床应用价值。
[Abstract]:Objective: to investigate the effects of periosteum distraction osteogenesis (periosteum distraction osteogenesis,PDO) combined with platelet-rich fibrin (platelet-rich fibrin,PRF) on mandibular alveolar ridge in rabbits. Materials and methods: Thirty-six adult New Zealand white rabbits were randomly divided into three groups: group A (n = 12) and group C (n = 12). All the experimental animals extracted the left mandibular first premolars and were included in the experiment one month later. Group A (PDO group): the left tooth extraction area was placed with distractor. Group B was treated with the same method of (PDO PRF): operation as group A. PRF membrane was placed on the surface of traction titanium mesh. Group C (heterotopic bone graft group): the left extraction area was implanted with 5*5*5mm xenograft, and the surface was covered by biofilm. The interval of 7 days after operation in group A was 7 days, and the rate of 0.25mm/ was 2 times a day. 6 animals were killed after 9 consecutive days of distraction, 60 days of fixation and 120 days of fixation. In group C, 6 animals were killed 60 days and 120 days after operation. To observe the new bone formation: gross observation, new bone height measurement, X-ray analysis, Micro-CT examination, non-decalcification hard tissue section observation, HE histological observation, BMP-2 immunohistochemical observation. Results: after 60 days of fixation in PDO group, some new bones were formed in the distraction zone and around the traction nail, the average height of the new bone was 2.65 卤0.37 mm, and the imaging showed that the middle and low density new bone could be seen under the titanium mesh and the structure was loose. Hard tissue sections and HE staining showed a large number of new trabeculae and active osteogenesis. After 120 days of fixation, the new bone under the titanium mesh tended to be mature and fused with the autogenous bone, the height of the new bone was 4.13 卤0.31 mm.Imaging showed that the new bone under the titanium mesh was high dense, the trabecular structure of the bone was clear, and the new bone fused with the surrounding alveolar bone. Histologically, the new bone tended to mature, and the mature Hafer system was observed. The results in group B were similar to those in group A. the grafts in group C were gradually absorbed and replaced by the new bone. Conclusion: the patented distractor was used for distraction of mandibular alveolar ridge periosteum in rabbits for 60 days. After 120 days of fixation, new bone formation was found in distraction space. PRF had no significant effect on PDO. PDO was simple and easy to operate, and no bone graft was needed. It has great clinical application value.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R782.1
本文编号：2359862
[Abstract]:Objective: to investigate the effects of periosteum distraction osteogenesis (periosteum distraction osteogenesis,PDO) combined with platelet-rich fibrin (platelet-rich fibrin,PRF) on mandibular alveolar ridge in rabbits. Materials and methods: Thirty-six adult New Zealand white rabbits were randomly divided into three groups: group A (n = 12) and group C (n = 12). All the experimental animals extracted the left mandibular first premolars and were included in the experiment one month later. Group A (PDO group): the left tooth extraction area was placed with distractor. Group B was treated with the same method of (PDO PRF): operation as group A. PRF membrane was placed on the surface of traction titanium mesh. Group C (heterotopic bone graft group): the left extraction area was implanted with 5*5*5mm xenograft, and the surface was covered by biofilm. The interval of 7 days after operation in group A was 7 days, and the rate of 0.25mm/ was 2 times a day. 6 animals were killed after 9 consecutive days of distraction, 60 days of fixation and 120 days of fixation. In group C, 6 animals were killed 60 days and 120 days after operation. To observe the new bone formation: gross observation, new bone height measurement, X-ray analysis, Micro-CT examination, non-decalcification hard tissue section observation, HE histological observation, BMP-2 immunohistochemical observation. Results: after 60 days of fixation in PDO group, some new bones were formed in the distraction zone and around the traction nail, the average height of the new bone was 2.65 卤0.37 mm, and the imaging showed that the middle and low density new bone could be seen under the titanium mesh and the structure was loose. Hard tissue sections and HE staining showed a large number of new trabeculae and active osteogenesis. After 120 days of fixation, the new bone under the titanium mesh tended to be mature and fused with the autogenous bone, the height of the new bone was 4.13 卤0.31 mm.Imaging showed that the new bone under the titanium mesh was high dense, the trabecular structure of the bone was clear, and the new bone fused with the surrounding alveolar bone. Histologically, the new bone tended to mature, and the mature Hafer system was observed. The results in group B were similar to those in group A. the grafts in group C were gradually absorbed and replaced by the new bone. Conclusion: the patented distractor was used for distraction of mandibular alveolar ridge periosteum in rabbits for 60 days. After 120 days of fixation, new bone formation was found in distraction space. PRF had no significant effect on PDO. PDO was simple and easy to operate, and no bone graft was needed. It has great clinical application value.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R782.1
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