姜黄素和Dickkopf-1联用对口腔鳞状细胞癌细胞增殖和凋亡的影响研究

发布时间：2019-03-13 10:44

【摘要】：目的:探讨姜黄素和分泌蛋白DKK1(Dickkopf-1)联用对口腔鳞状细胞癌细胞增殖和凋亡的影响。方法:CCK8实验检测0、5、10、20、40、80μmol/L的姜黄素作用于人口腔鳞状细胞癌CAL27、Tca8113、SCC-4细胞24、48、72h的细胞增殖情况,计算IC50值;实验分为对照组、姜黄素组、Dickkopf-1组、姜黄素+Dickkopf-1组,各组细胞处理48h后,CCK8试验检测细胞增殖,流式细胞仪检测细胞凋亡,Western blot检测Cleaved caspase3、β-catenin、CyclinD1蛋白表达。结果:不同浓度的姜黄素作用于CAL27、SCC-4、Tca8113细胞24、48、72h后的细胞抑制率均显著高于0h时的细胞抑制率,且随着时间延长及浓度增加细胞抑制率增加(P0.01),根据IC50值选择Tca8113细胞及30μmol/L的姜黄素作为后续研究。结论:姜黄素和Wnt/β-catenin信号通路抑制剂Dickkopf-1均能抑制口腔鳞状细胞癌细胞增殖和促进凋亡,两者联用的效果强于单独用姜黄素或Dickkopf-1。
[Abstract]:Objective: To study the effects of the combination of curcumin and the secretion protein DKK1 (Dickkopf-1) on the proliferation and apoptosis of oral squamous cell carcinoma cells. Methods: The cell proliferation of CAL27, Tca8113 and SCC-4 cells of human oral squamous cell carcinoma (CAL27), Tca8113 and SCC-4 cells (SCC-4) were measured by CCK8 and the IC50 values were calculated. The experiment was divided into the control group, the curcumin group, the Dickkopf-1 group, the curcumin + Dickkopf-1 group, and the cells were treated for 48 hours. The cell apoptosis was detected by flow cytometry, and the expression of Cleared caspase3,1-catenin and CyclinD1 was detected by Western blot. Results: The inhibitory rate of curcumin on CAL27, SCC-4, Tca8113 cells 24,48 and 72 h was significantly higher than that at 0 h, and the inhibition rate of cells increased with time and concentration (P0.01), and Tca8113 cells and 30. m u.mol/ L of curcumin were selected as a follow-up study according to the IC50 values. Conclusion: Both curcumin and Wnt/ HCO3-cattenin signal pathway inhibitor, Dickkopf-1, can inhibit the proliferation of oral squamous cell carcinoma cells and promote the apoptosis. The combination of both curcumin and Wnt/ HCO3-catenin signal pathway inhibitor is stronger than that of curcumin or Dickkopf-1 alone.
【作者单位】：清华大学医院口腔科;中国医学科学院北京协和医院口腔科;
【基金】：北京市自然科学基金资助项目(编号:7142135)
【分类号】：R739.8

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