口腔白斑组织中树突状细胞亚群的免疫组化分析

发布时间：2019-04-25 07:04

【摘要】：目的:分析口腔白斑(OLK)组织中树突状细胞的特征性表型。方法:应用免疫组化SP法检测10例正常黏膜、10例单纯增生白斑和20例异常增生白斑中树突状细胞特异性标记CD1a、CD209、CD123、CD83的表达状况,并进行统计学分析。结果:3组样本中CD1a、CD209均有表达。固有层内CD1a阳性郎格汉斯细胞(LCs)数量随病变程度逐渐升高,且异常增生白斑组高于单纯增生白斑组及正常黏膜组(P0.01)。异常增生白斑及单纯增生白斑中CD209阳性DCs被大量召集,其数量显著高于正常黏膜。正常黏膜中未见CD123及CD83表达,但在单纯增生及异常增生白斑中CD123阳性和CD83阳性细胞数逐渐升高(P0.01)。结论:朗格汉斯细胞、间质DC及浆细胞样树突细胞在OLK间质的表达水平增加逐渐成熟与机体的免疫监视功能密切相关,在OLK发展过程中均可能起到重要作用。
[Abstract]:Objective: to analyze the characteristic phenotype of dendritic cells in oral leukoplakia (OLK) tissue. Methods: immunohistochemical SP method was used to detect the expression of dendritic cell-specific CD1a,CD209,CD123,CD83 in 10 cases of normal mucosa, 10 cases of simple hyperplasia leukoplakia and 20 cases of dysplasia leukoplakia. Results: CD1a,CD209 was expressed in all three groups. The number of CD1a-positive Langerhans cells in the lamina propria increased gradually with the degree of lesion, and the number of (LCs) in dysplasia leukoplakia group was higher than that in simple hyperplastic leukoplakia group and normal mucous membrane group (P0.01). The number of CD209-positive DCs in dysplasia leukoplakia and simple hyperplasia leukoplakia was significantly higher than that in normal mucosa. There was no expression of CD123 and CD83 in normal mucosa, but the number of CD123 positive and CD83 positive cells increased gradually in simple hyperplasia and dysplasia leukoplakia (P0.01). Conclusion: the increased expression of Langerhans cells, interstitial DC and plasmacytoid dendritic cells in the stroma of OLK is closely related to the immune monitoring function of the body, and may play an important role in the development of OLK.
【作者单位】：汕头大学医学院;北京大学深圳医院口腔颌面外科;
【基金】：广东省自然科学基金(编号:S2012010010382) 深圳市创新委国际合作(编号:ZYA201106100080A);深圳市创新委基础研究(编号:JCY20130402114702120)
【分类号】：R739.85

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