生长激素对大鼠正畸牙齿移动过程中骨桥蛋白表达的影响

发布时间：2019-04-28 07:04

【摘要】：目的:研究生长激素对正畸牙齿移动过程中牙根周围组织中骨桥蛋白(osteopontin,OPN)表达的影响。方法:将40只2月龄体重约200 g雄性Wistar大鼠随机分成对照组和实验组,每组各20只。建立大鼠上颌第一磨牙正畸牙齿移动模型。实验组大鼠予0.15 U/(kg.d)的生长激素腹部皮下注射;对照组大鼠每天注射等量的生理盐水。于大鼠上颌中切牙和左侧上颌第一磨牙之间放置镍钛拉簧,拉力为0.49 N,使磨牙向中切牙方向移动。加力后的第3,7,10,14天,用心脏灌注法处死所有大鼠。取下左侧上颌骨,制备第一磨牙近远中向的纵向切片,免疫组织化学染色观察近颊根与远颊根之间牙槽骨的压力区中牙周膜OPN的表达,并进行半定量分析。结果:实验组和对照组的成骨细胞、破骨细胞和骨细胞的胞质中都有OPN的阳性表达,并且有相同的变化趋势,OPN阳性表达早期随破骨细胞的增多而增加,后期随破骨细胞的减少而降低。正畸加力后第7天,与对照组相比,实验组OPN阳性表达更强(P0.05);第10天,与对照组相比,实验组OPN阳性表达下降更快,实验组明显低于对照组(P0.05)。结论:全身应用生长激素能影响大鼠移动牙齿时牙周组织中OPN的表达,可能对正畸牙齿移动过程中压力侧骨的吸收发挥重要的调节作用。
[Abstract]:Aim: to investigate the effect of long hormone on the expression of osteopontin (osteopontin,OPN) in periroot tissue during orthodontic tooth movement. Methods: forty 2-month-old male Wistar rats were randomly divided into two groups: control group (n = 20) and experimental group (n = 20). The orthodontic tooth movement model of maxillary first molar was established in rats. The rats in the experimental group were subcutaneously injected with 0.15U / (kg.d) of growth hormone, and the rats in the control group were injected with the same amount of saline every day. A Ni-Ti pull spring was placed between the maxillary middle incisor and the left maxillary first molar. The tensile force was 0.49 N, which made the molar move toward the middle incisor. At days 3, 7, 10, 14, all rats were killed by cardiac perfusion. The left maxillary bone was removed and the proximal and distal longitudinal sections of the first molar were prepared. Immunohistochemical staining was used to observe the expression of periodontal ligament OPN in the pressure region of the alveolar bone between the proximal and distal buccal roots, and semi-quantitative analysis was carried out. Results: there was positive expression of OPN in osteoblasts, osteoclasts and osteoblasts in both experimental and control groups. The expression of OPN in osteoblasts, osteoclasts and osteoblasts in the early stage increased with the increase of osteoclasts. At the later stage, the number of osteoclasts decreased with the decrease of osteoclasts. On the 7th day after orthodontic treatment, the positive expression of OPN in the experimental group was stronger than that in the control group (P0.05); on the 10th day, the positive expression of OPN in the experimental group decreased faster than that in the control group, and was significantly lower in the experimental group than that in the control group (P0.05). Conclusion: growth hormone can affect the expression of OPN in periodontal tissue during tooth movement in rats, and may play an important role in regulating the bone resorption of pressure side during orthodontic tooth movement.
【作者单位】：山西医科大学汾阳学院口腔科;
【分类号】：R783.5

【相似文献】