BAMBI在小鼠不同类型脂肪组织发育及脂肪细胞分化过程中的差异表达规律

发布时间：2018-05-03 16:52

本文选题：BAMBI + RT-qPCR　；参考：《南方医科大学学报》2015年01期

【摘要】：目的研究BAMBI基因在小鼠不同脂肪组织发育过程中的表达规律。方法利用RT-q PCR及Western blot技术检测BAMBI基因在小鼠不同类型脂肪组织、不同发育阶段(胚胎第18天、出生第0天、第21天、第8周和第6个月)及原代前体脂肪细胞分化过程中(分化第0、3、7、11天)的差异表达情况。结果随着脂肪组织的发育,BAMBI m RNA及蛋白的表达量逐渐降低;在发育的同一时间段,BAMBI m RNA在内脏白色脂肪组织较皮下白色脂肪组织表达量高,在棕色脂肪组织中表达量最低;在小鼠前体脂肪细胞诱导分化过程中,BAMBI m RNA表达量呈时间依赖性降低。结论提示BAMBI的差异表达规律与脂肪组织类型、发育阶段以及脂肪细胞分化程度密切相关,其在脂肪组织发育过程中起着重要的作用。
[Abstract]:Objective to study the expression of BAMBI gene in different adipose tissues of mice. Methods RT-q PCR and Western blot techniques were used to detect the BAMBI gene in different types of adipose tissues of mice at different stages of development (18th day of embryo, day 0 of birth, day 21 of birth). At the 8th week and 6th month, the differential expression of preadipocytes was observed during the differentiation of the primary adipocytes (the 0th day of differentiation). Results with the development of adipose tissue, the expression of BAMBI m RNA and protein decreased gradually, and the expression of BAMBI m RNA in visceral white adipose tissue was higher than that in subcutaneous white adipose tissue, and the lowest in brown adipose tissue. The expression of BAMBI m RNA decreased in a time dependent manner during the differentiation of mouse precursor adipocytes. Conclusion the differential expression of BAMBI is closely related to adipose tissue type, development stage and adipocyte differentiation, which plays an important role in adipose tissue development.
【作者单位】：西安交通大学医学部生理学与病理生理学系;教育部环境与疾病相关基因重点实验室;西安交通大学第一附属医院血液内科;Metabolic
【基金】：国家自然科学基金(31200886) 中央高校基本科研业务费专项资金~~
【分类号】：R363

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