弹性蛋白酶诱导兔囊性动脉瘤模型的建立及其病理学研究

发布时间：2018-05-24 15:40

本文选题：动脉瘤 + 动物模型　；参考：《南昌大学》2009年硕士论文

【摘要】： 目的:应用猪胰弹性蛋白酶消化法建立兔囊性动脉瘤模型,观察该模型的形态学和病理学变化。方法:应用猪胰弹性蛋白酶消化颈总动脉建立兔右颈总动脉起始部的囊性动脉瘤模型, MRA检查动态观察该模型3天、14天、28天动脉瘤形态学变化, HE染色和弹性纤维EVG染色法,光镜和电镜下观察该模型0天、14天、28天动脉瘤的病理学变化。结果:动脉瘤模型制作成功后早期动脉瘤进行性增大,14天后大小趋于稳定。动脉瘤模型病理学观察发现0天动脉瘤模型血管内皮细胞消失,瘤壁弹力层完全缺失, 28天后动脉瘤壁逐渐增厚,仍缺乏弹力层,但胶原纤维增生。结论:猪胰弹性酶消化法建立的兔囊性动脉瘤模型,不仅形态和病理上与人类颅内动脉瘤相似,而且早期具有自发生长的特性,提示该模型不仅可用于研究血管内栓塞材料的实验研究,也可用于动脉瘤形成机制的研究。
[Abstract]:Objective : To establish a model of rabbit cystic aneurysm by porcine pancreatic elastase digestion and to observe the morphological and pathological changes of the model .

Methods : The saccular aneurysm model of right common carotid artery was established with porcine pancreatic elastase . MRA was used to observe the morphological changes of aneurysm morphology , HE staining and EVG staining in the model , HE staining and EVG staining . The pathological changes of aneurysm were observed on 0 day , 14 days and 28 days under light microscope and electron microscope .

Results : The aneurysm model was more progressive after successful operation , and the size of aneurysm was stable after 14 days . The aneurysm model pathology observed that the vascular endothelial cells disappeared and the elastic layer of tumor wall was completely absent . After 28 days , the wall of aneurysm was gradually thickened , and the elastic layer was still lacking , but the collagen fibers were proliferated .

Conclusion : The rabbit cystic aneurysm model established by porcine pancreatic elastase is not only similar to human intracranial aneurysm but also spontaneous growth in early stage , suggesting that the model not only can be used to study the experimental study of endovascular embolization material , but also can be used in the study of aneurysm formation mechanism .
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R543.5;R-332

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