精索静脉曲张大鼠睾丸附睾损害模型复制的研究

发布时间：2018-05-29 12:08

本文选题：精索静脉曲张 + 睾丸附睾损害　；参考：《山东中医药大学》2009年硕士论文

【摘要】： 目的:通过复制精索静脉曲张大鼠睾丸附睾损害模型,为进一步药物干预的研究提供实验基础。方法:Wistar青春期雄性大鼠48只,随机分为两组:模型组36只,对照组12只。模型组使用Saypol等的方法即部分结扎左肾静脉法复制精索静脉曲张模型,对照组暴露左肾静脉过程相同,但不结扎。8周后从模型组随机抽取12只连同对照组一同处死,观察两组大鼠睾丸重量及睾丸、附睾镜下形态学改变。结果:造模8周后,大鼠睾丸重量模型组左侧与右侧、模型组左侧与对照组两侧均有显著性差异(P＜0.05)。同时,镜下观察模型组双侧睾丸及附睾均有明显的病理损害。结论:精索静脉曲张大鼠左侧睾丸重量明显变小,双侧睾丸、附睾有严重的病理形态学改变,说明精索静脉曲张大鼠睾丸附睾损害模型复制成功。
[Abstract]:Objective: to provide experimental basis for further drug intervention by replicating the damage model of testicular epididymis in rats with varicocele. Methods: 48 male Wistar male rats were randomly divided into two groups: the model group 36 and the control group 12. The model group was partially ligated the left renal vein to copy the varicocele pattern with the method of ligation of the left renal vein. In the control group, the left renal vein was exposed to the same process, but after.8 weeks, 12 rats were randomly selected from the model group and executed together with the control group. The two groups of rats' testis weight and testicles and the morphological changes under the epididymis microscope were observed. Results: after 8 weeks of modeling, the rat testis weight model group was left and right, the left side of the model group and the two sides of the control group were significant. The sex difference (P < 0.05). At the same time, there were obvious pathological damage to both testis and epididymis in the model group. Conclusion: the left testicular weight of the varicocele rats was obviously smaller, and the bilateral testicles and epididymis had serious pathological changes, which showed that the testis epididymal damage model of the varicocele rats was successfully replicated.
【学位授予单位】：山东中医药大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R-332

【参考文献】