热量限制对大鼠下丘脑和垂体组织电压依赖性钙通道表达的调节作用

发布时间：2018-06-04 06:03

本文选题：热量限制 + 下丘脑　；参考：《华中科技大学》2008年硕士论文

【摘要】： 目的:上世纪90年代以来发现热量限制可以延长线虫,果蝇及大鼠的寿命,而机体衰老最突出的表现之一就是神经内分泌系统功能紊乱,作为和内分泌系统关系最为密切的中枢神经组织下丘脑和垂体,执行着把神经系统和内分泌系统信号联系在一起的重要功能。电压依赖性钙通道在激素分泌和激素对中枢神经系统信号传递过程中均起到了极为重要的作用。为此本课题通过研究热量限制大鼠的下丘脑和垂体组织中钙通道的基因表达变化,来揭示神经内分泌的联动作用,进而阐明热量限制延缓衰老在神经与内分泌方面的机制。方法:建立热量限制的大鼠CR模型,动物分为2组,分为给予随意饮食即(Ad libitum, AL)组和60%饲料的(Calorie restriction, CR)组,限食8个月后测量大鼠体重变化及血清中超氧化物歧化酶(SOD)、丙二醛(MDA)的水平,采用实时定量PCR技术(real-time PCR)、免疫印迹技术(Western blotting)和免疫组织化学方法检测下丘脑和垂体组织中L型、T型、N型、R型和P/Q型五种钙通道亚型的基因表达变化。结果:经过8个月热量限制,CR大鼠体重明显低于AL组;MDA水平CR组较AL组明显减少,SOD活性两组无明显差别;五种钙通道的基因和蛋白表达均有所下降,在下丘脑,N型、R型有统计学差异,在垂体N型、T型和R型有统计学差异。结论:CR后大鼠下丘脑和垂体的L型、T型、N型、R型和P/Q型五种钙通道的mRNA水平和蛋白表达均有所下调,在长期饥饿应激过程中,leptin,insulin,orexin,ghrelin的水平发生改变,而这些激素的分泌和释放与钙通道密切相关,进而可能在长期应激中改变了通道的基因表达。
[Abstract]:Objective: since 1990s, caloric restriction has been found to prolong the life span of nematodes, Drosophila melanogaster and rats, and one of the most prominent signs of aging is the dysfunction of neuroendocrine system. The hypothalamus and pituitary, which are most closely related to the endocrine system, play an important role in linking the signals of the nervous system and the endocrine system. Voltage-dependent calcium channel plays an important role in hormone secretion and hormone signaling in the central nervous system. Therefore, by studying the changes of calcium channel gene expression in hypothalamus and pituitary tissues of rats with calorific restriction, this study will reveal the neuroendocrine interaction and clarify the neuroendocrine mechanism of caloric restriction in delaying aging. Methods: cr model was established in rats with caloric restriction. The rats were divided into two groups: ad libitum (ALL) group and calorie restriction (CR) group, which were fed 60% diet. The changes of body weight and the levels of serum superoxide dismutase (SOD) and malondialdehyde (MDA) in rats were measured after 8 months of food restriction. Real-time PCR technique was used to detect the gene expression of five calcium channel subtypes in hypothalamus and pituitary tissues. Results: after 8 months of caloric restriction, the body weight of CR rats was significantly lower than that of AL group. There was no significant difference in SOD activity between CR group and AL group, and the gene and protein expression of five calcium channels were decreased. There was statistical difference in N type R type in hypothalamus and T type and R type in pituitary gland. Conclusion the expression of mRNA and protein in L type, T type, N type, R type and P / Q type calcium channels in hypothalamus and pituitary were down-regulated, and the level of leptin insulininorexinghrelin was changed during the long period of starvation stress. The secretion and release of these hormones are closely related to calcium channels, which may alter the gene expression of these channels during long-term stress.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R33

【共引文献】