IL-37抑制脂多糖诱导的小鼠树突状细胞活化

发布时间：2018-06-04 06:44

本文选题：白细胞介素 + 树突状细胞　；参考：《细胞与分子免疫学杂志》2015年04期

【摘要】：目的探讨白细胞介素37(IL-37)对细菌脂多糖(LPS)诱导的小鼠树突状细胞(DC)活化的调节作用。方法应用GM-CSF和IL-4诱导小鼠骨髓细胞向DC分化,抗CD11c磁珠分选DC。IL-37预处理DC后,进行LPS刺激。流式细胞术检测DC表面共刺激分子(CD80、CD86)表达水平,实时荧光定量PCR检测肿瘤坏死因子α(TNF-α)、IL-6和IL-1αmRNA表达水平,流式细胞微球芯片试剂盒(CBA试剂盒)检测细胞培养上清中IL-1α、IL-6、TNF-α等因子的浓度。结果 DC诱导成功,磁珠分选能够获得高纯度的DC(90%)。IL-37降低LPS诱导的DC表面共刺激分子CD80、CD86的表达,并抑制DC合成IL-1α、IL-6、TNF-α。结论 IL-37可以通过降低共刺激分子和炎症因子的表达抑制LPS刺激的DC活化。
[Abstract]:Objective to investigate the effect of interleukin 37 (IL 37) on the activation of murine dendritic cells (DC) induced by lipopolysaccharide (LPS). Methods GM-CSF and IL-4 were used to induce the differentiation of mouse bone marrow cells into DC. The DC was pretreated by anti-CD11c magnetic beads and then stimulated by LPS. Flow cytometry was used to detect the expression of costimulatory molecule CD80 (CD86) on DC surface, real-time quantitative PCR was used to detect the expression of TNF- 伪 TNF- 伪, IL-6 and IL-1 伪 mRNA, and the concentration of IL-1 伪, IL-6 and TNF- 伪 in cell culture supernatant was detected by flow cytometry. Results DC was induced successfully. DC(90%).IL-37 with high purity could reduce the expression of CD80 CD86 on DC surface induced by LPS, and inhibit the synthesis of IL-1 伪 -IL-6 TNF- 伪 by DC. Conclusion IL-37 can inhibit DC activation induced by LPS by decreasing the expression of costimulatory molecules and inflammatory factors.
【作者单位】：中国医学科学院北京协和医学院血液病医院血液学研究所;天津市东丽区东丽医院;天津医科大学总医院消化科;
【基金】：国家重大科学研究计划(2013CB966904) 国家自然科学基金(81273217) 天津市应用基础及前沿技术研究计划(12JCYBJC32800) 高等学校博士学科点专项科研基金(20121106120037)
【分类号】：R392

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