星形胶质细胞纤维生长和突触形成的方向性和特异性

发布时间：2018-06-05 03:51

本文选题：星形细胞 + 突触　；参考：《中国组织工程研究》2015年42期

【摘要】：背景:星形胶质细胞除了被动地起代谢和结构支持的作用外,还能够以更复杂的方式与神经元相互作用,主动地参与中枢神经系统的信息处理。目的:通过观察星形胶质细胞纤维生长和突触形成的方向性和特异性,探索星形胶质细胞在与神经元相互作用中的可能机制。方法:取新生24 h内绿色荧光蛋白转基因小鼠,以原代细胞分离培养和爬片方法获得海马原代细胞,以胶质原纤维酸性蛋白荧光免疫组化方法特异性标记星形胶质细胞,在共聚焦显微镜下观察结果。结果与结论:星形胶质细胞纤维向距离较远的锥体细胞定向伸长,并且终止于锥体细胞的胞体,末端与锥体细胞的胞体形成突触样结构;而且并不是所有的突起都伸向临近的细胞,其突触样结构的形成也不全是在最临近的位点。表明星形胶质细胞纤维伸长和突触形成具有方向性和特异性,为进一步探索星形胶质细胞与锥体细胞之间的信号传导提供依据。
[Abstract]:Background: in addition to the role of passive metabolism and structural support, astrocytes can interact with neurons in a more complex way and participate actively in the information processing of the central nervous system. Aim: to explore the possible mechanism of astrocyte interaction with neurons by observing the directionality and specificity of astrocyte fiber growth and synapse formation. Methods: Hippocampal primary cells were obtained from transgenic mice with green fluorescent protein (GFP) within 24 hours after birth. Astrocytes were specifically labeled with glial fibrillary acidic protein (GFAP) fluorescence immunohistochemical method. The results were observed under confocal microscope. Results and conclusion: the astrocyte fibers extended to the pyramidal cells which were far away and terminated in the pyramidal cells. The terminals of astrocytes formed synaptic structures with the pyramidal cells. Moreover, not all the processes extend to the adjacent cells, and the synaptophysin formation is not always at the nearest site. The results suggest that the fiber elongation and synapse formation of astrocytes are directional and specific, which provides a basis for further exploration of signal transduction between astrocytes and pyramidal cells.
【作者单位】：首都医科大学附属北京天坛医院临床医学研究实验室;
【基金】：国家自然科学基金(81303268,81572474) 北京市自然科学基金(7132094,7152098,7144209)~~
【分类号】：R338

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