兔眼外源性烟曲霉性眼内炎模型建立

发布时间：2018-06-07 10:25

本文选题：烟曲霉 + 眼内炎　；参考：《吉林大学》2008年硕士论文

【摘要】： 目的:建立兔眼外源性烟曲霉性眼内炎模型。方法:健康成年新西兰大白兔24只,随机分为8组,其中7组为实验组,另一组为对照组。实验组分别于右眼玻璃体腔内接种浓度为(1.0-1.5)×101、102、103、104、105、106、107 CFU/ml的烟曲霉孢子悬液各0.1ml,对照组右眼玻璃体腔内注射0.9%生理盐水0.1ml。采用直接检眼镜进行真菌性眼内炎自然病程及临床特征观察15天,处死后取玻璃体腔液、血液及全身主要脏器进行真菌培养,进行真菌生态学及分子生物学鉴定,并将眼球作组织学检查。选取能引起典型临床特征的最低浓度孢子悬液进行重复实验。结果:(1.0-1.5)×104CFU/ml组可引起眼部组织的典型感染症状。结论:成功建立兔眼外源性烟曲霉性眼内炎模型。
[Abstract]:Objective: to establish the model of exogenous aspergillus fumigatus endophthalmitis in rabbit eyes. Methods: 24 healthy adult New Zealand white rabbits were randomly divided into 8 groups, 7 of them were experimental group and the other was control group. The experimental group was inoculated with 0.1 ml Aspergillus fumigatus spore suspension at the concentration of 1.0-1.5) 脳 101102103104105106107 CFU/ml in the vitreous cavity of the right eye, and the control group was injected 0.9% normal saline 0.1 ml into the vitreous cavity of the control group. The natural course and clinical characteristics of fungal endophthalmitis were observed by direct ophthalmoscope for 15 days. The vitreous cavity fluid, blood and main organs of the whole body were taken for fungal culture, and the fungal ecology and molecular biological identification were carried out. Histological examination of the eyeball was made. The lowest concentration spore suspensions which can cause typical clinical characteristics were selected for repeated experiments. Results 1. 0-1. 5) 脳 104CFU/ml group could cause typical infection symptoms of ocular tissue. Conclusion: the model of exogenous aspergillus fumigatus endophthalmitis was successfully established in rabbit eyes.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R771;R-332

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