三种Hib结合疫苗的免疫原性研究

发布时间：2018-06-07 19:29

本文选题：Hib结合疫苗 + 免疫原性　；参考：《重庆医科大学》2010年硕士论文

【摘要】： 目的:以大鼠为模型,对市场上常见的三种Hib结合疫苗的免疫原性进行研究,比较三种疫苗产生的抗体水平,了解几种疫苗之间免疫原性是否具有差别,为儿童选择接种Hib疫苗提供可靠的依据,为将Hib疫苗纳入计划免疫提供一定的基础。方法:选择SPF级5天龄的SD大鼠共128只,清洁级动物间母乳喂养,21天龄左右离乳。将5d龄乳鼠随机分组,共四组。包括实验组3组(贺新立适组、安尔宝组、云南玉溪组),35只/组,对照组(生理盐水组)1组,23只/组。在5d,19d,26d龄时给予相应疫苗(生理盐水)20μl皮下免疫注射。获得初次免疫前及末次免疫后1周,2周,4周的血清。使用ELISA法测定各组血清中Hib IgG抗体。比较每组注射疫苗前后的抗体水平,比较各组间的抗体水平。结果:实验组疫苗注射前后抗体浓度比较,差异性显著,末次免疫后1周时抗体浓度均较免疫前明显增高(p0.01),第2周时抗体浓度继续升高(p0.01),第4周时有明显下降趋势(p0.01);对照组不同时间抗体浓度无明显差异(p0.01)。各组间抗体浓度比较,其结果如下:免疫前组间抗体水平不存在显著性差异(p0.01)。末次免疫后1周,各实验组与对照组间均存在显著性差异(p0.01),贺新立适组抗体浓度最高,与安尔宝组无明显差异(p0.01),与云南组间存在显著性差异(p0.01),安尔宝组与云南组差异不显著(p0.01);末次免疫后2周及4周,贺新立适组抗体浓度较高,与安尔宝组、云南组之间均存在显著性差异(p0.01),安尔宝组与云南组差异不显著(p0.01)。结论:三种Hib结合疫苗均具有良好的免疫原性;三种Hib疫苗产生的抗体浓度变化趋势大致相同,在不同抗体水平基础上,均在免疫后第1周有抗体产生,第2周时继续增高,第4周有明显的下降。贺新立适产生的抗体始终处于较高水平,安尔宝与国产Hib疫苗无显著差异。免疫接种时推荐优先使用贺新立适,可作为儿童Hib预防接种的良好选择。
[Abstract]:Objective: to study the immunogenicity of three Hib conjugated vaccines in the market in rats, compare the antibody levels of the three vaccines and find out whether there are differences in the immunogenicity of the three vaccines. Methods: 128 SD rats of SPF grade 5 days old were selected and breast-fed between clean grade animals about 21 days old. Five day old neonatal rats were randomly divided into four groups. There were 35 rats in the experimental group (Hexinlishi group, Anerbao group, Yuxi group, Yunnan province) and 23 rats in the control group (normal saline group). The corresponding vaccine (20 渭 l saline) was injected subcutaneously at the age of 26 days. The serum was obtained before the first immunization and 1 week and 4 weeks after the last immunization. Hib IgG antibody in serum of each group was determined by Elisa. The antibody levels of each group before and after vaccination were compared. Results: the concentration of antibody in the experimental group was significantly different before and after the injection of vaccine. After the last immunization, the antibody concentration was significantly higher at 1 week than that before immunization, and continued to increase at the second week, and decreased significantly at the 4th week, but there was no significant difference between the control group and the control group at different time points (P 0.01). The results were as follows: there was no significant difference in antibody level between groups before immunization (p 0.01). One week after the last immunization, there was a significant difference between the experimental group and the control group (p0.01), and the highest antibody concentration was found in the Hexinlsuo group. There was no significant difference between Anerbao group and Yunnan group, but there was no significant difference between Anerbao group and Yunnan group (P 0.01), but there was no significant difference between Anerbao group and Yunnan group (P 0.01), and the concentration of antibody in Hexinlite group was higher than that in Anerbao group at 2 and 4 weeks after the last immunization. There was significant difference between Yunnan group and Yunnan group, but there was no significant difference between Anerbao group and Yunnan group. Conclusion: all the three Hib conjugated vaccines have good immunogenicity, and the change trend of antibody concentration of the three Hib vaccines is about the same. On the basis of different antibody levels, antibodies were produced at the first week after immunization, continued to increase at the second week, and decreased significantly at the fourth week. The antibody produced by he Xinlishi was always at a high level, and there was no significant difference between the vaccine of Albo and Hib vaccine made in China. Hexinlite is recommended as a good choice for Hib vaccination in children.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R392

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