创伤愈合过程中血管新生模式研究

发布时间：2018-06-14 23:34

本文选题：创伤愈合 + 血管新生模式　；参考：《苏州大学》2009年硕士论文

【摘要】： 毛细血管是唯一进行代谢物质交换的场所,为机体各器官提供营养物质,氧气及置换代谢产物CO_2等,并传送出体外。当局部组织发生创伤后,毛细血管的新生既是组织自我修复完整性的一部分,也是组织进一步修复的基础条件。新毛细血管的形成对供养新近形成的组织是必需的。血管新生可以缩短氧从毛细血管向细胞弥散的距离,增加细胞氧的供应,从而改善细胞在缺氧条件下的功能,并进而改善整个创伤组织的供氧情况。出芽和套叠是创伤愈合过程中的血管新生的主要方式,虽然创伤愈合中血管新生的研究已取得了诸多进展,对套叠和出芽血管新生的具体过程、基本机制有了较多的了解,但对创伤愈合中血管新生详细的机制仍然未被阐明。为了探索这些问题的答案,本研究试图通过提出毛细血管网络构建的一系列假设,并依据内皮细胞因子向周围组织扩散的模型,对内皮细胞对于不同浓度的内皮生长因子的反应进行研究,以期探讨血管新生的模式。在本文中主要通过以下几个方面的工作开展本课题的研究。一、对创伤愈合中的血管新生研究的背景及研究对象相关的认知现状与基础知识的梳理。由于血管新生伴随着创伤愈合的整个过程,对创伤愈合的基本过程进行了介绍。并详细说明了关于血管新生与创伤愈合的关系的研究。同时对近年来创伤愈合中的血管新生数学模型的研究进行了总结,提出这些模型存在的问题,提出本论文研究的目的和意义。二、分析与探讨出芽新生和套迭新生的基本过程以及各自的调控机制,在出芽新生和套迭新生研究的基础上,关注创伤愈合过程中的血管新生的机制和模式。三、通过实验观察毛细血管在创伤愈合中不同阶段的生长形态。在成年SD大鼠皮肤做深至全层的切割伤,在创伤后不同时相点取材,通过透射电镜观察毛细血管和内皮细胞的形态,来推测血管的新生模式。通过实验发现在创伤修复的过程中,创伤早期急性缺氧条件下毛细血管主要以出芽新生。在缺氧程度减缓的情况下,VEGF浓度下降,套迭新生出现。四、基于创伤部位或缺氧源部位的细胞大量表达VEGF;血管内皮细胞受体Flk-1和Flt-1增加;VEGF与受体特异性结合,促进内皮细胞的分裂增殖;VEGF浓度不同,内皮细胞反应不同等事实,提出毛细血管网络建构的一些假设,建立内皮生长因子向周围组织扩散的模型,并对影响内皮生长因子浓度的因素进行分析和讨论,根据内皮细胞对不同浓度的内皮生长因子的应答,构建毛细血管的新生及其网络形成模式。通过这些研究,我们对于伤口愈合中毛细血管生长的模式与内皮细胞对不同浓度的内皮生长因子的反应之间的关系提出了新的看法与模型,为获得判断伤口愈合中的血管新生的方式的依据问题进行了探索。
[Abstract]:Capillaries are the only places where metabolites are exchanged, which provide nutrients, oxygen and replacement metabolites COSP 2 for various organs of the body, and transport them out of the body. When local tissue is traumatized, capillary neovascularization is not only a part of tissue self-repair integrity, but also a basic condition for further tissue repair. The formation of new capillaries is necessary to support newly formed tissue. Angiogenesis can shorten the distance of oxygen diffusion from capillaries to cells, increase the supply of oxygen to cells, thus improve the function of cells under hypoxia, and then improve the oxygen supply of the whole wound tissue. Budding and intussusception are the main ways of angiogenesis in wound healing. Although many advances have been made in the study of angiogenesis in wound healing, the basic mechanism of angiogenesis in the process of intussusception and budding has been well understood. However, the detailed mechanism of angiogenesis in wound healing remains unexplained. In order to explore the answers to these questions, this study attempts to propose a series of assumptions for the construction of capillary networks, and based on the model of endothelial cytokine diffusion to surrounding tissues. The response of endothelial cells to different concentrations of endothelial growth factor (et) was studied in order to explore the pattern of angiogenesis. In this paper, mainly through the following aspects of work to carry out the study of this subject. First, the background of angiogenesis research in wound healing and the related cognitive status and basic knowledge. The basic process of wound healing is introduced because angiogenesis is accompanied by the whole process of wound healing. The relationship between angiogenesis and wound healing is described in detail. At the same time, the research of angiogenesis mathematical models in wound healing in recent years is summarized, the problems existing in these models are put forward, and the purpose and significance of this paper are put forward. Secondly, the basic process and regulation mechanism of budding and overlaying are analyzed and discussed. Based on the research of budding and overlaying, the mechanism and mode of angiogenesis in wound healing are paid more attention to. Third, the growth of capillaries in different stages of wound healing was observed experimentally. In adult SD rats, the skin was cut deep to the whole layer, and the morphology of capillary and endothelial cells was observed by transmission electron microscope (TEM) to predict the pattern of angiogenesis. It was found that in the process of wound repair, the capillary mainly sprout in the early stage of trauma under the condition of acute hypoxia. Under the condition of decreasing hypoxia, the concentration of VEGF decreased and the overlap appeared. (4) VEGF was expressed in large quantities in the cells of wound site or anoxic source, the vascular endothelial cell receptors Flk-1 and Flt-1 increased the specific binding of VEGF to the receptor, and promoted the proliferation of endothelial cells. The concentration of VEGF was different and the response of endothelial cells was different. Some hypotheses of capillary network construction were put forward, and a model of endothelial growth factor diffusion to surrounding tissues was established, and the factors affecting the concentration of endothelial growth factor were analyzed and discussed. According to the response of endothelial cells to different concentrations of endothelial growth factor, the capillary neovascularization and its network formation model were constructed. Through these studies, we have proposed a new view and model of the relationship between the capillary growth pattern in wound healing and the response of endothelial cells to different concentrations of endothelial growth factor. In order to obtain the basis for judging the way of angiogenesis in wound healing.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R641;R363

【引证文献】