重组禽流感H5蛋白表位疫苗的表达、纯化及免疫原性研究

发布时间：2018-06-18 00:53

本文选题：重组疫苗 + 禽流感　；参考：《吉林大学》2009年硕士论文

【摘要】： 高致病性禽流感(HPAI)病毒,特别是H5N1毒株,可以在饲养和野生的鸟类种群有很高的发病率与死亡率,在最近的暴发流行导致了严重的经济损失。亚洲和欧洲相继有H9N2、H7N7和H5N1亚型禽流感病毒感染人类的报道,而且疫情有日益严重的趋势。2009年以来,我国数个省区陆续有人感染禽流感的报告。 HA(凝血素蛋白)不但在病毒分类及变异中是主要标志,在抗病毒感染中也有很重要的作用,在现有的对禽流感病毒感染的免疫机制研究中认为,HA蛋白是介导病毒进入宿主细胞的重要配体分子,因此抗HA蛋白的中和抗体在阻断HA介导病毒进入细胞内增殖的过程中具有重要的作用。HA是禽流感病毒的重要抗原成分,进入机体后可引起特异性的免疫应答,可避免再次接触同种病毒时的感染。因此,诱导机体形成对HA蛋白的特异性免疫是预防禽流感的一个重要目标。HA蛋白还可能是抗病毒药物作用的靶点。本研究的目的就是用原核系统重组H5蛋白,使其能够模拟天然H5蛋白诱导有效的针对H5亚型禽流感病毒的免疫,在刘旭等人工作的基础上,进一步完善了重组H5蛋白L3的表达条件、纯化方案,建立了较稳定的间接ELISA实验体系检测L3蛋白作为抗原检测相应抗体和灭活病毒作为抗原检测相应抗体,并且根据交叉反应原理来评估重组蛋白L3与天然蛋白H5在免疫原性上的差异,初步评估重组蛋白是否可以作为新型禽流感疫苗应用。
[Abstract]:The highly pathogenic avian influenza virus (HPAI), especially the H5N1 strain, can cause high morbidity and mortality in breeding and wild bird populations, resulting in serious economic losses in recent outbreaks. In Asia and Europe, human infection with H9N2FH7N7 and H5N1 subtype avian influenza virus has been reported, and the epidemic has become increasingly serious. In several provinces and regions of China, there have been reports of human infection with avian influenza. HAA (hemagglutinin protein) is not only the main marker of virus classification and mutation, but also plays an important role in anti-virus infection. In the current studies on the immune mechanism of avian influenza virus infection, it is considered that the HA protein is an important ligand that mediates virus entry into host cells. Therefore, the neutralizing antibody against HA protein plays an important role in blocking the proliferation of virus into cells mediated by HA. Ha is an important antigen component of avian influenza virus, and it can cause a specific immune response after entering the body. Can avoid the infection of contact with the same virus again. Therefore, inducing the formation of specific immunity to HA protein is an important target for the prevention of avian influenza. Ha protein may also be a target of antiviral drugs. The aim of this study is to recombine H5 protein with prokaryotic system, so that it can mimic the natural H5 protein to induce effective immunization against H5 subtype avian influenza virus, based on the work of Liu Xu et al. The expression conditions and purification schemes of recombinant H5 protein L3 were further improved. A stable indirect Elisa system was established to detect L3 protein as antigen and inactivated virus as antigen to detect corresponding antibody. The immunogenicity of recombinant protein L3 and natural protein H5 was evaluated according to the principle of cross reaction, and whether the recombinant protein could be used as a new avian influenza vaccine was preliminarily evaluated.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R392

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