西北寒燥证证候模型的建立及其生物学基础的相关研究

发布时间：2018-06-18 01:48

本文选题：寒燥环境 + 生物表征　；参考：《新疆医科大学》2009年硕士论文

【摘要】： 目的:研究西北干燥寒冷环境对小鼠生物表征的的影响,并揭示这种环境对机体产生影响的部分生物学基础,为西北寒燥证证候模型的建立及评价提供思路。方法:根据中医病因学说理论,用人工气候箱模拟新疆特有的干燥寒冷环境,并作用于ICR小鼠,观察记录小鼠各种生物表征的变化,在HE染色光镜和透射电镜下分别观察小鼠各脏器组织形态和细胞超微结构的改变,用高效液相色谱-电化学检测法测定小鼠脑组织和外周血中单胺类神经递质的变化。结果:和空白对照组相比,置于寒燥环境中的小鼠除出现一般寒、燥表现外,中期毛发稀疏少泽,逐渐变得烦躁、易怒,修饰反应增加,对刺激反应敏感,出现撕咬打斗现象;后期渐趋于安静,对刺激表现较前迟钝,毛色暗淡无光泽。所提取原色和亮度方面,以舌尖部的明显变化出现最早,舌根部最晚;小鼠的爪、尾所提取原色和亮度数值的变化与舌的变化趋势有着某种程度的一致性,而且爪与舌的相似度优于尾与舌的相似度;前期两组小鼠体重相比差异明显,后期这种差异不具统计学意义;小鼠21天的进食量和饮水量和空白对照组相比有明显差异。寒燥环境组与空白对照组相比各种脏器在组织形态和超微结构上有明显改变。寒燥环境应激21天小鼠血清中的去甲肾上腺素、多巴胺明显低于对照组(P 0.01)五羟色胺高于对照组(P 0.05),而脑中去甲肾上腺素、多巴胺和5-羟色胺含量均明显高于对照组(P 0.01)。结论:1、寒燥环境可引起小鼠各种生物表征的改变,且这种变化有着时程性;2、寒燥环境可能是西北寒燥证形成的主要原因;3、西北寒燥证不是某一脏器的改变,而是机体整体性、全身性、系统性的改变;4、寒燥证证候模型的建立,给沙漠周边居民、石油工人、来疆民工及驻疆解放军、武警指战员等特殊人群的异常生物表征改变提供了部分生物学基础研究的支撑,为下一步治疗及预防工作的开展提供了实验支持;5、小鼠望诊,可增加望爪和望尾两部分。
[Abstract]:Objective: To study the effect of dry and cold environment on the biological characterization of mice in Northwest China, and to reveal the biological basis of the effect of this environment on the organism, and to provide ideas for the establishment and evaluation of the northwest cold dryness syndrome model. Method: according to the theory of TCM etiology, the special dry and cold environment of Xinjiang is simulated with a human working climate box. The changes of various biological characteristics of mice were observed and recorded in ICR mice. The changes of tissue morphology and ultrastructure of various organs of mice were observed under HE staining and transmission electron microscopy. The changes of monoamine neurotransmitters in the brain tissue and peripheral blood of mice were measured by high performance liquid chromatography electrochemical detection. Results: and blank control In the cold dry environment, the mice in the cold environment were not only cold and dry, but the middle hair was thinning and less, gradually became irritable, irritable, the reaction was increased, the reaction was sensitive to the stimulus, and the phenomenon of tearing and fighting appeared, and the later Yu Anjing was gradually dull and dull and dull. The obvious change of the tip was the earliest, the root of the tongue was the latest. The change of the original color and brightness values of the mice's claw and tail had some consistency with the change trend of the tongue, and the similarity between the tongue and the tongue was better than the similarity between the tail and the tongue; the two groups of mice in the early stage were obviously different in weight, and the difference was not statistically significant in the later period. Compared with the blank control group, the food intake and the amount of drinking water were significantly different between the 21 days and the blank control group. The cold dryness environment group and the blank control group had obvious changes in the tissue morphology and ultrastructure. The norepinephrine in the serum of 21 days in the cold dryness environment was significantly lower than the control group (P 0.01) and five HT was higher than the control group (P 0.05), the content of norepinephrine, dopamine and 5- hydroxytryptamine in the brain was significantly higher than that in the control group (P 0.01). Conclusion: 1, cold dryness environment can cause changes in various biological characterization of mice, and this change has time history; 2, cold dryness environment may be the main cause of the formation of northwest cold dryness syndrome; 3, northwest cold dryness syndrome is not a change of a certain organ, It is the integral, systemic and systematic change of the body; 4, the establishment of a cold dryness syndrome model provides support for the abnormal biological characterization of special populations around the desert surrounding residents, oil workers, migrant workers and the PLA, the armed police officers and other special groups of people, and for the next step of treatment and prevention. It provides experimental support. 5, the inspection of the mice can increase the two parts of the claw and the tail.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R256;R-332

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