江西地区汉族人群6个STR基因座位群体遗传多态性研究

发布时间：2018-06-21 23:50

本文选题：短串联重复序列(STR) + 聚合酶链反应(PCR)　；参考：《南昌大学》2009年硕士论文

【摘要】： 目的:调查江西地区汉族人群无关个体D6S1043、D2S1772、D7S3048、D22-GATA198B05、D8S1132与D11S2368等6个STR基因座的遗传多态性,积累群体遗传学数据,探讨其应用价值。方法:采集江西地区212例汉族无关个体EDTA抗凝全血,应用STR-PCR与PAGE检测D6S1043、D2S1772、D7S3048、D22-GATA198B05、D8S1132与D11S2368等6个STR基因座的等位基因。结果:在212例江西地区汉族人群中观察到D6S1043基因座位13个基因,52种基因型;D2S1772基因座13种基因,66种基因型;D7S3048基因座位12种基因,48种基因型;D22-GATA198B05基因座位11种基因,44种基因型;D8S1132基因座10种基因,38种基因型;D11S2368基因座位10种基因41种基因型。D6S1043、D2S1772、D7S3048、D22-GATA198B05、D11S2368与D8S1132等6个基因座的观察杂合度(h)分别为:0.8019、0.8302、0.8538、0.8302、0.8774与0.8443.期望杂合度(H)分别为:0.8727、0.8896、0.8813、0.8553、0.8559、0.8611;个人识别率(DP)分别为:0.9662、0.9735、0.9697、0.9607、0.9559、0.9613;非父排除率(PE)分别为:0.7394、0.7751、0.7445、0.7086、0.7053、0.7219;多态信息含量(PIC)分别为:0.8475、0.8774、0.8730、0.8453、0.8452、0.8510。6个位点的累积非父排除率(CPE)为0.0000000001,累积个人识别率(CDP)为0.9996423770。结论:本次调查6个STR位点在江西地区汉族人群的分布符合Hardy-Weinberg平衡,遗传多态性高。本次调查为丰富江西地区遗传资料库及实现本地区法医学亲子鉴定和个人识别提供了基础数据和理论依据。
[Abstract]:Objective: To investigate the genetic polymorphism of 6 independent STR loci, such as D6S1043, D2S1772, D7S3048, D22-GATA198B05, D8S1132 and D11S2368, in the Han population of Jiangxi, and to accumulate the population genetic data and explore its application value.
Methods: EDTA anticoagulant blood was collected from 212 unrelated individuals of Han nationality in Jiangxi. The alleles of 6 STR genes, such as D6S1043, D2S1772, D7S3048, D22-GATA198B05, D8S1132 and D11S2368, were detected by STR-PCR and PAGE.
Results: 13 genes, 52 genotypes, 13 genes, 66 genotypes, 12 D7S3048 gene seats, 48 genotypes, 11 gene loci, 44 genotypes of D22-GATA198B05, 10, 38 and D11S2368 genes of the D8S1132 loci were observed in 212 Jiangxi Han population. The heterozygosity (H) of 6 loci.D6S1043, D2S1772, D7S3048, D22-GATA198B05, D11S2368 and D8S1132, respectively, were: 0.8019,0.8302,0.8538,0.8302,0.8774 and 0.8443. expected heterozygosity (H) respectively: 0.8727,0.8896,0.8813,0.8553,0.8559,0.8611, and the individual recognition rate (DP) was respectively: 0.9607,0.9559,0.9613, the non parent exclusion rate (PE) was 0.7394,0.7751,0.7445,0.7086,0.7053,0.7219, and the polymorphic information content (PIC) was: the cumulative non parent exclusion rate (CPE) of 0.8475,0.8774,0.8730,0.8453,0.8452,0.8510.6 sites was 0.0000000001, and the cumulative individual recognition rate (CDP) was 0.9996423770..
Conclusion: the distribution of 6 STR loci in the Jiangxi Han population in this survey accords with the balance of Hardy-Weinberg and high genetic polymorphism. This survey provides basic data and theoretical basis for enriching the genetic database in Jiangxi area and realizing the paternity and personal identification of forensic medicine in the region.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R394

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