焦虑和社会支持对临床实验指标的影响及其与HLA-DQB1等位基因的关联

发布时间：2018-06-22 00:44

本文选题：焦虑 + 社会支持　；参考：《大连医科大学》2010年硕士论文

【摘要】： 目的：本研究通过对不同人群焦虑和社会支持情况的调查,以及临床实验指标的检测,旨在了解受心理因素影响而变化的临床实验指标,并在分子生物学水平探讨这种心理因素对机体生理的影响及其与HLA-DQB 1基因之间的关联性。方法： 1.选取三组正常健康人群(医生31人,大学生51人,农民51人)共133人,发放状态—特质焦虑问卷和社会支持评定量表,测量其焦虑和社会支持情况,并检测其血常规,血清生化以及体液免疫指标。以状态-特质焦虑问卷和社会支持评定量表的评分做为分组标准,得分最高的40人(男女各20人)作为高分组,最低的40人(男女各20人)作为低分组,通过比较两组之间各项检测指标的统计学差异,探讨受焦虑和社会支持影响而变化的临床实验指标。 2.采用PCR-SSP技术,检测医生人群的HLA-DQB 1等位基因,并结合其焦虑评分和临床实验指标,探讨HLA-DQB1等位基因对焦虑引起的临床实验指标变化的影响。结果： 1.焦虑和社会支持之间的负性相关具有统计学意义,三组人群之间的焦虑和社会支持情况的差异均具有统计学意义。 2.焦虑和社会支持高分组和低分组之间的某些血常规及血清生化指标之间的差异具有统计学意义(P0.05),状态焦虑高分组相较于低分组,MCV.RDWSD降低；特质焦虑高分组相较于低分组,NEUT#.MCV.MCH降低；客观支持高分组相较于低分组,MPV升高,而RDWSD.LDL降低；主观支持高分组相较于低分组,RDWSD.TP.ALB.A/G.Chol. HDL.LDL升高,MONO％.GLU降低；支持利用度高分组相较于低分组,RDWSD.TP.A/G升高,而MCHC降低,支持总分高分组相较于低分组,RDWSD.A/G升高,而MONO%.GLU.TP.ALB.Chol.LDL. hs-CRP降低。 3.HLA-DQB1*02位点对状态焦虑下LYMPH#.MCV.BUN.hs-CRP.IgA、补体C3的变化有显著影响；对特质焦虑下PLT.PCT的变化有显著影响。HLA-DQB1*04对状态焦虑下MCV.ALB.A/G.BUN.hs-CRP.补体C3的变化有显著影响；对特质焦虑下MCV.TP.BUN.hs-CRP.补体C3的变化有显著影响。HLA-DQB1*06位点对特质焦虑下IgM的变化有显著影响。结论：焦虑和社会支持对机体某些临床实验指标的变化有显著影响,而这种影响可能与HLA-DQB1等位基因*02、,*04、*06位点有关。
[Abstract]:Objective: the purpose of this study was to investigate the anxiety and social support of different population groups, as well as to investigate the clinical experimental indexes, which were influenced by psychological factors. At the level of molecular biology, the influence of this psychological factor on organism physiology and its association with HLA-DQB1 gene were discussed. Methods: 1. Three groups of healthy people (31 doctors, 51 college students, 51 farmers) were selected. The state trait anxiety questionnaire and the social support rating scale were issued to measure their anxiety and social support, and their blood routine was tested. Serum biochemical and humoral immune indexes. The scores of state trait anxiety questionnaire and social support rating scale were used as the criteria, the highest 40 (20 male and 20 male) as the high score group, the lowest 40 (20 male and 20 male) as the low group. By comparing the statistical differences between the two groups, we discussed the clinical experimental indexes which were influenced by anxiety and social support. 2. The HLA-DQB1 allele was detected by PCR-SSP technique, and the influence of HLA-DQB1 allele on the changes of clinical experimental indexes induced by anxiety was investigated. Results: 1. The negative correlation between anxiety and social support was statistically significant, and the differences of anxiety and social support among the three groups were statistically significant. 2. There were significant differences in some blood routine and serum biochemical indexes between the high score group and the low group (P0.05). The MCV.RDWSD of the high anxiety group was lower than that of the low group, and the trait anxiety group was lower than that of the lower group (NEUT#.MCV.MCH). The MPV of high score group was higher than that of low group, while RDWSD.LDL was lower than that of high score group, and that of high score group was higher than that of low group, and that of high score group was higher than that of low group, and that of RDWSD.TP.ALB.A / G. Chol. HDL.LDL increased and MONO.GLU decreased; the RDWSDL. TP.Ar. G increased in the high score group compared with the low group, while the MCHC decreased, and the RDWSDL. Ar. G increased in the high score group compared with the low group. However, MONO.GLU.TP.ALB.Chol.LDL.hs-CRP decreased. 3. HLA-DQB1O02 had a significant effect on the changes of LYMPH#.MCV.BUN.hs-CRP.IgA, complement C3 under state anxiety, and on the changes of PLT.PCT under trait anxiety. The changes of complement C3 had a significant effect on MCV.TP.BUN.hs-CRP under trait anxiety. The change of complement C3 had a significant effect on the change of IgM under trait anxiety. Conclusion: anxiety and social support have significant effects on the changes of some clinical experimental parameters, and this effect may be related to the HLA-DQB1 allelic gene (Z02QB1).
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R395.1

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