雄性兔低顺应性膀胱模型的建立

发布时间：2018-06-24 16:14

本文选题：膀胱出口梗阻 + 低顺应性　；参考：《天津医科大学》2010年硕士论文

【摘要】： 目的：通过兔膀胱出口部分梗阻的办法,建立低顺应性膀胱动物模型,观察梗阻后至低顺应性膀胱形成的过程中膀胱重量、膀胱容量、逼尿肌漏尿点压力及顺应性的变化,研究其发展变化的机制和重要环节,以期在膀胱功能发生不可逆改变之前,缓解膀胱出口梗阻后膀胱储尿功能的改变。方法： 28只雄性新西兰兔随机分成3组：对照组8只(A组),梗阻5周组10只(B组),梗阻14周组10只(C组)。梗阻5周组和14周组用相同手术方法建立膀胱出口部分梗阻模型：取耻骨上正中切口,游离膀胱颈,避开膀胱两侧韧带内的血管,钝性游离膀胱颈,以2-0丝线自后方带出结扎膀胱颈。对照组只做游离,不结扎。梗阻5周组于5周时进行尿动力学检测,C组分别于8周、12周、14周进行尿动力学检测,各组检测完成后处死并留取膀胱标本,常规HE染色观察膀胱壁平滑肌,纤维结缔组织变。结果：膀胱重量梗阻5周组(16.9±4.4)g,梗阻14周组膀胱重量(14.1±2.3)g,明显高于对照组(2.7±0.5)g,均有统计学差异(P0.01)。膀胱容量梗阻5周(67.0±17.3)ml、梗阻8周(57.8±16.9)ml与对照组(36.3±14.7)m1相比显著增加(P0.01),梗阻后14周膀胱容量(20.9±6.2)ml小于对照组,有统计学差异(P0.05)。梗阻12周、14周膀胱容量与梗阻5周、8周相比显著减少(P0.01)。膀胱顺应性梗阻5周(5.16±1.41)m1／cmH2O、梗阻8周(4.39±1.72)cm／H2O与对照组(2.91±0.71)ml／cmH2O比明显升高(P0.01,P0.05),梗阻12周膀胱顺应性(1.53±0.49)ml／cmH2O低于对照组(P0.05),梗阻14周组膀胱顺应性(1.21±0.44)ml／cmH2O与对照组比显著下降(P0.01)。结论：通过兔膀胱颈不全结扎法建立的BOO实验动物模型具有方法简便、存活率高、稳定性好等特点,适用于膀胱出口梗阻的相关实验研究。膀胱出口梗阻14周后膀胱顺应性明显下降,低顺应性膀胱建立成功。膀胱出口梗阻5周、8周、12周、14周后膀胱结构和功能发生了不同程度的改变：膀胱重量显著增加,逼尿肌漏尿点压逐渐增加,膀胱容量先增加后明显减少,膀胱顺应性呈现先增高后降低的趋势。
[Abstract]:Objective: to establish an animal model of low compliance bladder by using the method of partial bladder outlet obstruction in rabbits, and to observe the bladder weight and bladder capacity during the process of bladder formation from obstruction to low compliance. The changes of detrusor leakage point pressure and compliance were studied to study the mechanism and important link of detrusor leakage point pressure and compliance in order to alleviate the change of bladder urine storage function after bladder outlet obstruction before the irreversible change of bladder function. Methods: Twenty-eight male New Zealand rabbits were randomly divided into three groups: control group (n = 8), obstruction group (n = 10), obstruction group (n = 10) and group C (n = 10). The model of bladder outlet partial obstruction was established in the 5th and 14th week groups with the same surgical method: the suprapubic median incision, the free bladder neck, avoiding the vessels in the ligaments of both sides of the bladder, and the blunt free bladder neck. The bladder neck was ligated with 2-0 thread from the rear. In the control group, only free, no ligation. Urodynamic examination was performed in group C at 5 weeks after obstruction. The bladder specimens were sacrificed and collected after 8 weeks and 12 weeks and 14 weeks respectively. The smooth muscle of bladder wall and connective tissue were observed by routine HE staining. Results: the weight of bladder in 5 weeks group and 14 week group was (16.9 卤4.4) g and (14.1 卤2.3) g respectively, which was significantly higher than that in control group (2.7 卤0.5) g (P0.01). The bladder volume at 5 weeks after obstruction (67.0 卤17.3) ml and at 8 weeks (57.8 卤16.9) ml was significantly higher than that in the control group (36.3 卤14.7) ml (P0.01). The bladder volume at 14 weeks after obstruction was (20.9 卤6.2) ml lower than that in the control group (P0.05). The bladder volume at 12 and 14 weeks after obstruction was significantly lower than that at 5 and 8 weeks after obstruction (P0.01). Bladder compliance was (5.16 卤1.41) ml / cm H _ 2O at 5 weeks and (4.39 卤1.72) cm / h _ 2O at 8 weeks after obstruction compared with (2.91 卤0.71) ml / cm H _ 2O in control group (P 0.05). At 12 weeks of obstruction, bladder compliance was (1.53 卤0.49) ml / cm H _ 2O lower than that in control group (P0.05), and bladder compliance was (1.21 卤0.44) ml 路cmH _ 2O lower than that in control group at 14 weeks (P0.01). Conclusion: the Boo animal model established by incomplete ligation of bladder neck in rabbits has the advantages of simple method, high survival rate and good stability. It is suitable for the experimental study of bladder outlet obstruction. After 14 weeks of bladder outlet obstruction, bladder compliance decreased significantly, and low compliance bladder was successfully established. After 5 weeks, 8 weeks, 12 weeks and 14 weeks of bladder outlet obstruction, the bladder structure and function changed to different degrees: bladder weight increased significantly, detrusor leakage point pressure increased gradually, bladder capacity increased first and then decreased significantly. Bladder compliance increased first and then decreased.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R-332

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