血管性痴呆大鼠模型两种复制方法的比较

发布时间：2018-06-24 20:33

本文选题：血管性痴呆 + 动物模型　；参考：《湖南中医药大学学报》2015年01期

【摘要】：目的探讨血管性痴呆动物模型制备的有效方法。方法跳台实验选取Wister雄性大鼠60只,随机数字表法分为传统法模型组、改良法模型组、假手术组,每组20只。传统法模型组参照Farkas E等的双侧颈总动脉结扎法。改良法模型组在Farkas E等的基础上取去尖长度40 mm,直径0.3 mm的天协牌针灸针与颈总动脉同时结扎,之后抽去针灸针,缝合切口。观察两组大鼠的存活率、学习记忆能力改变、病理形态学变化。结果术后7 d,传统法组存活7只,改良法组存活17只;术后4、8 w,两组的逃避潜伏期缩短,穿越平台错误次数增多,两组间均无显著性差异(P0.05),两组的海马区神经元有相似程度的变性。结论两种方法均可复制血管性痴呆模型,改良法较传统法的死亡率明显降低。
[Abstract]:Objective to explore an effective method for the preparation of vascular dementia animal model. Methods Sixty Wister male rats were randomly divided into traditional model group, modified model group and sham operation group with 20 rats in each group. The traditional model group referred to the bilateral common carotid artery ligation with Farkas E et al. On the basis of Farkas E et al, the modified model group removed 40 mm of tip length and 0.3 mm diameter of Tianxie acupuncture needle and ligated the common carotid artery simultaneously, then removed the acupuncture needle and sutured the incision. The survival rate, learning and memory ability and pathomorphology of the two groups were observed. Results 7 days after operation, 7 rats survived in the traditional method group and 17 in the modified method group, and the escape latency shortened and the number of platform errors increased in both groups at 4 to 8 weeks after operation. There was no significant difference between the two groups (P0.05), the hippocampal neurons of the two groups had the same degree of degeneration. Conclusion both methods can replicate vascular dementia model and the mortality rate of the modified method is significantly lower than that of the traditional method.
【作者单位】：安徽中医药大学;
【基金】：国家自然科学基金资助课题(81373744)
【分类号】：R-332;R749.13

【共引文献】