小鼠肝癌原位移植模型的建立及其意义的研究

发布时间：2018-06-24 20:50

本文选题：肝癌 + H细胞　；参考：《黑龙江医药科学》2015年06期

【摘要】：目的:探讨用肝癌H22细胞建立小鼠肝癌原位移植模型。方法:将肝癌H22细胞注入小鼠腹腔内,建立异位移植模型;抽取腹水中癌细胞接种于小鼠肝实质内,建立原位移植模型。结果:移植后10d可扪及肿瘤生长,模型成功率94.4%;晚期自发转移率79.4%,腹水产生率35.3%,无自发消退;移植瘤保持甲胎蛋白(AFP)、异常凝血酶原(DCP)高分泌及γ-氨基酰转氨酶(γ-GT)同工酶阳性的特点;模型平均自然生存期28d。结论:小鼠肝癌原位模型成功率高,易于制作,生物学特征符合模拟人类肝癌在体内发生发展的全过程,可成为今后人类研究肝癌的重要动物模型。
[Abstract]:Objective: to study the orthotopic transplantation model of liver cancer in mice with hepatoma H 22 cells. Methods: the hepatoma H22 cells were injected into the abdominal cavity of mice to establish the model of heterotopic transplantation, and the cancer cells in ascites were extracted and inoculated into the liver parenchyma of mice to establish the orthotopic transplantation model. Results: the tumor growth was palpable 10 days after transplantation, the success rate of the model was 94. 4%, the rate of late spontaneous metastasis was 79. 4%, the rate of ascites production was 35. 3%, and there was no spontaneous regression. The transplanted tumor maintained the characteristics of alpha-fetoprotein (AFP), abnormal prothrombin (DCP) hypersecretion and 纬 -aminotransferase (纬 -GT) isozyme, and the average natural survival time of the model was 28 days. Conclusion: the mouse hepatoma in situ model has a high success rate and is easy to be made. The biological characteristics are in line with the whole process of the development of human liver cancer in vivo and can be used as an important animal model for human liver cancer research in the future.
【作者单位】：佳木斯大学附属第一医院普外二科;
【基金】：佳木斯大学研究生科技创新项目,编号:LM2014-018
【分类号】：R735.7;R-332

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