X线辐射对大鼠卵巢形态与功能的影响

发布时间：2018-06-24 21:06

本文选题：X线 + 辐射　；参考：《国际妇产科学杂志》2011年05期

【摘要】：目的:探讨X线电离辐射对大鼠卵巢形态与功能的影响。方法:实验分0,0.2,0.4,0.6,0.8,1.0Gy剂量组,每组各10只大鼠。采用HE染色观察各级卵泡数目,免疫组化法观察P53、Bcl-2蛋白表达,TUNEL法测定颗粒细胞中凋亡小体的表达。结果:随大鼠机体接受X线照射剂量增加,始基卵泡、初级卵泡、次级卵泡数目随之减少(P0.05或P0.01),以始基卵泡减少为著;不同剂量X线照射后窦状卵泡数目与假照射组差异无统计学意义(P0.05)。当吸收剂量为0.2Gy,卵巢颗粒细胞中P53蛋白及凋亡小体表达较假照射组减少,Bcl-2蛋白有所增多(P0.05或P0.01),而吸收剂量在0.4～1.0Gy时,P53蛋白及凋亡小体的表达水平随吸收剂量的增加而上升(P0.05或P0.01),Bcl-2蛋白表达水平减少(P0.01)。结论:X线可使大鼠卵巢组织中始基卵泡的数目有所减少,促进细胞发生凋亡,进一步影响颗粒细胞的内分泌功能。
[Abstract]:Objective: to investigate the effects of X-ray ionizing radiation on ovarian morphology and function in rats. Methods: the experiment was divided into 0 ~ (0.2) ~ 0. 4 ~ 0. 4 ~ 0. 6 and 0. 8 ~ 0. 0 Gy dose group with 10 rats in each group. The number of follicles was observed by HE staining and the expression of P53 Bcl 2 protein was detected by immunohistochemical method. The expression of apoptotic corpuscles in granulosa cells was detected by Tunel method. Results: the number of primary follicles, primary follicles and secondary follicles decreased with the increase of irradiation dose (P0.05 or P0.01). There was no significant difference in the number of antral follicles between the two groups after X ray irradiation (P0.05). When the absorbed dose was 0.2 Gy, the expression of p53 protein and apoptotic corpuscles in ovarian granulosa cells decreased significantly (P0.05 or P0.01) compared with those in false irradiation group. However, the expression level of p53 protein and apoptotic corpuscles in ovarian granulosa cells at the dose of 0.4 渭 g / 1.0 Gy was increased with the absorbed dose (P0.05 or P0.01). Increased and increased (P0.05 or P0.01) the expression level of Bcl-2 protein decreased (P0.01). Conclusion the number of primordial follicles in ovarian tissue of rats was decreased, apoptosis was promoted and the endocrine function of granulosa cells was further affected by% X ray.
【作者单位】：武警医学院附属医院妇产科;
【分类号】：R737.31

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