26RFa及其C末端片段在心血管和痛觉方面活性研究

发布时间：2018-07-01 20:57

本文选题：26RFa + C末端片段　；参考：《兰州大学》2009年硕士论文

【摘要】： 26RFa是新发现的RFamide结尾的相关肽,其内源性受体为GPR103。结构分析发现26RFa C末端八肽高度保守,并且中间存在螺旋结构引领着N-末端和C-末端。已有的研究表明26RFa相关肽能介导多种生理活性,主要包括摄食,能量消耗,运动,内分泌,骨生成和痛觉调节。但现在对26RFa生理活性研究的还不全。本论文主要选择26RFa、C-末端片段26RFa(19-26),26RFa(8-26)作为GPR103受体激动剂来探讨他们对大鼠心血管系统影响和小鼠痛觉系统影响及其作用机制。研究表明静脉注射26RFa(100-800 nmol/kg,i.v.)可以使麻醉的大鼠血压双向变化并增加心率,升血压和心率过程依赖儿茶酚胺机制;侧脑室注射大鼠/小鼠26RFa(5,10 and 20nmol)有明显的镇痛作用,是通过抑制下行γ-氨基丁酸能神经元起作用,与阿片系统无关。26RFa的两个C-末端片段26RFa(8-26)和26RFa(19-26)在外周心血管活性和脊髓水平上镇痛调节等方面的作用可能是通过与26RFa作用途径不同的其它机制起作用的,从而暗示了N-末端对于26RFa生物活性的维持可能起着重要作用。
[Abstract]:26RFa is a newly discovered peptide associated with the end of RFamide, and its endogenous receptor is GPR103. Structural analysis showed that the C-terminal octapeptide of 26RFa was highly conserved, and there was a helical structure in the middle leading the N-terminal and C-terminal. Previous studies have shown that 26RFa-related peptides mediate a variety of physiological activities, including food intake, energy expenditure, exercise, endocrine, bone formation and pain regulation. However, the physiological activity of 26RFa has not been fully studied. In this study, we selected 26RFa (19-26) 26RFa (8-26) as the agonist of GPR103 receptor to investigate their effects on the cardiovascular system in rats and the pain system in mice and its mechanism. The results showed that 26RFa (100-800 nmol / kg i.v.) was injected intravenously. The changes of blood pressure in anesthetized rats were bidirectional and increased heart rate. The mechanism of ascending blood pressure and heart rate was dependent on catecholamine mechanism, and intracerebroventricular injection of 26RFa (5 ~ 10 and 20nmol) had obvious analgesic effect. By inhibiting downlink 纬 -aminobutyric acid neurons, The effects of two C-terminal fragments 26RFa (8-26) and 26RFa (19-26) unrelated to the opioid system on peripheral cardiovascular activity and analgesic regulation at spinal cord level may be mediated by other mechanisms different from those of 26RFa. This suggests that N- terminal may play an important role in the maintenance of 26RFa biological activity.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R33

【相似文献】