β-溶血性链球菌红霉素耐药特征与大环内酯类耐药基因的分析

发布时间：2018-07-02 07:07

本文选题：β-溶血性链球菌 + 红霉素　；参考：《中国抗生素杂志》2015年09期

【摘要】：目的了解安徽地区β-溶血性链球菌大环内酯类耐药基因的携带情况,并与红霉素药敏结果进行对比分析。方法对来源于安徽地区2005—2013年临床分离的61株β-溶血性链球菌采用琼脂稀释法测定红霉素的最小抑菌浓度(MIC),同时应用PCR扩增大环内酯类相关耐药基因。结果 61株β-溶血性链球菌中A群(Group A Streptococcus,GAS)占21.3%,B群(Group B Streptococcus,GBS)占68.8%,C和G群(Group C Streptococcus,GCS、Group G Streptococcus,GGS)共占9.9%,A群及B群对红霉素的耐药率较高,分别为76.9%和85.7%,携带的耐药基因均以erm C基因(76.9%和66.7%)占主导。1株GCS对红霉素耐药且只携带erm B基因,GGS对红霉素的耐药率为60%,以erm B和erm C基因的检出率最高,均为60%。结论安徽地区β-溶血性链球菌的红霉素耐药率较高,且主要由erm基因介导的重度耐药常见。临床医生应加强耐药监测,依据药敏结果指导用药。
[Abstract]:Objective to investigate the carrying status of 尾-hemolytic streptococcus macrolide resistance gene in Anhui province and to compare the results with erythromycin susceptibility. Methods the minimal inhibitory concentration (MIC) of erythromycin was determined by Agar dilution method in 61 strains of 尾 -hemolytic streptococcus isolated from 2005 to 2013 in Anhui area. Meanwhile, macrolide related drug resistance genes were amplified by PCR. Results Group A Streptococcus gas (Group A Streptococcus gas) and Group B Streptococcus GBS (Group C Streptococcus Group G Streptococcus GGS) accounted for higher resistance to erythromycin than Group A (Group A) and Group G (Group C Streptococcus sp. GGS). 76.9% and 85.7% of the resistant genes were erm C gene (76.9% and 66.7%), respectively. The resistance rate of erm B gene to erythromycin was 60%, and the detection rate of erm B and erm C gene was both 605%. Conclusion the erythromycin resistance rate of 尾-hemolytic streptococcus in Anhui is high, and severe resistance mediated mainly by erm gene is common. Clinicians should strengthen the monitoring of drug resistance and guide the drug use according to the results of drug sensitivity.
【作者单位】：安徽医科大学第一附属医院感染病科;安徽省细菌耐药监测中心;
【基金】：安徽省自然科学基金(No.1408085QH176)
【分类号】：R378

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