新西兰大白兔髓核组织在自身椎管内组织学和免疫学改变的研究

发布时间：2018-07-13 07:30

【摘要】： 目的：制作游离型腰椎间盘突出动物模型,观察并探讨游离髓核组织重吸收的可能机制,为临床保守治疗某些类型腰椎间盘突出症提供新的思路和依据。设计、时间、地点：随机对照动物实验,2009年10月-2010年3月在延边大学解剖学教研部动物实验室及延边大学医院临床病理科完成。材料：健康雄性新西兰大白兔90只,随机分为三组：模型组60只,假模型组24只,对照组6只。方法：模型组前方入路制作游离型腰椎间盘突出动物模型,假模型组取相同手术入路,但仅暴露至纤维环而不做后续处理,对照组不做任何处理。模型组及假模型组分别于术后2、4、6、8、10、12周分批处死动物,取手术区间椎间盘标本。用免疫组化方法观察髓核组织中CD68+巨噬细胞浸润情况。结果：模型组移植的髓核组织不同程度缩小甚至消失,所有移植标本内均见CD68+巨噬细胞浸润随时间延长而逐渐减少,假模型组仅纤维环外缘见少量CD68+巨噬细胞浸润,其髓核及对照组髓核均未见CD68+巨噬细胞浸润。结论：(1)运用前方手术入路可建立游离型腰椎间盘突出动物模型。(2)游离髓核组织在硬膜外腔可逐渐被机体重吸收。(3)CD68+巨噬细胞浸润在游离髓核组织重吸收过程中起到重要作用。
[Abstract]:Objective: to make an animal model of free lumbar intervertebral disc herniation, observe and explore the possible mechanism of reabsorption of free nucleus pulposus tissue, so as to provide new ideas and basis for the conservative treatment of some types of lumbar intervertebral disc herniation.
Design, time, place: randomized controlled animal experiments, completed in March -2010 October 2009 in the animal laboratory of the Department of anatomy, Yanbian University and the clinicopathological Department of the Yanbian University hospital.
Materials: 90 healthy male New Zealand white rabbits were randomly divided into three groups: model group 60, false model group 24 and control group 6.
Methods: the animal model of the free lumbar intervertebral disc herniation was made in front of the model group. The false model group was taken the same surgical approach, but only the fibrous ring was exposed without subsequent treatment. The control group did not do any treatment. The model group and the fake model group died at the 2,4,6,8,10,12 weeks after the operation, and the intervertebral disc specimens were taken from the operation interval. Histochemical method was used to observe the infiltration of CD68+ macrophages in nucleus pulposus.
Results: the nucleus pulposus tissue of the model group reduced and even disappeared in different degrees. The infiltration of CD68+ macrophage in all the transplanted specimens gradually decreased with time. Only a small amount of CD68+ macrophage infiltration in the false model group was seen in the outer margin of the false model group, and the nucleus pulposus and the nucleus pulposus of the control group had no CD68+ macrophage infiltration.
Conclusions: (1) an animal model of free lumbar intervertebral disc herniation can be established by using the anterior approach. (2) the free nucleus pulposus can be gradually absorbed by the body in the epidural cavity. (3) the infiltration of CD68+ macrophages plays an important role in the reabsorption of free nucleus pulposus tissue.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R329

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